Functional Analysis of DNA and Chromatin
Editors: Stockert, Juan Carlos, Espada, Jesús, Blázquez-Castro, Alfonso (Eds.)
Free Preview- Presents an overview of standard and more recent methods for the functional analysis of the genetic material
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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In Functional Analysis of DNA and Chromatin, expert researchers in the field provide an overview of standard and more recent methods for the functional analysis of the genetic material. These include methods on DNA-dye binding modes, chromatin staining, nuclear dispersion assays, DNA labeling in vivo, sister chromatid exchanges, FISH, DNA and chromatin imaging by fluorescence, electron and atomic force microscopy, detection of apoptotic DNA, cytosine methylation and hydroxy-methylation, DNA thermophoresis, improved methods for histone analysis, chromatin immunoprecipitation, and analysis of rDNA genes and chromatin-associated RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Functional Analysis of DNA and Chromatin seeks to aid scientists in the further study of cellular and molecular biology of the nucleus.
- Table of contents (28 chapters)
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Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern
Pages 1-12
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Using Microchip Gel Electrophoresis to Probe DNA–Drug Binding Interactions
Pages 13-24
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Identifying Different Types of Chromatin Using Giemsa Staining
Pages 25-38
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Analysis of DNA Damage and Repair by Comet Fluorescence In Situ Hybridization (Comet-FISH)
Pages 39-48
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Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level
Pages 49-70
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Table of contents (28 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Functional Analysis of DNA and Chromatin
- Editors
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- Juan Carlos Stockert
- Jesús Espada
- Alfonso Blázquez-Castro
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1094
- Copyright
- 2014
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, New York
- Distribution Rights
- Distribution rights for India: CBS Publishers, New Delhi, India
- eBook ISBN
- 978-1-62703-706-8
- DOI
- 10.1007/978-1-62703-706-8
- Hardcover ISBN
- 978-1-62703-705-1
- Softcover ISBN
- 978-1-4939-6048-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 365
- Number of Illustrations
- 30 b/w illustrations, 44 illustrations in colour
- Topics
