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Brain Development

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Simon G Sprecher

    1. Department of Biology, University of Fribourg, Fribourg, Switzerland

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  • Introduces cutting-edge techniques in developmental neurobiology through genetic model systems as well as some complimentary animal models
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and expert implementation advice

Part of the book series: Methods in Molecular Biology (MIMB, volume 1082)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-x
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  2. Drosophila Protocols

    1. Front Matter

      Pages 1-1
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    2. Immunostaining of the Developing Embryonic and Larval Drosophila Brain

      • Danielle C. Diaper, Frank Hirth
      Pages 3-17

    3. Non-fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila

      • David Jussen, Rolf Urbach
      Pages 19-35

    4. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling

      • Karoline F. Kraft, Rolf Urbach
      Pages 37-56

    5. Flybow to Dissect Circuit Assembly in the Drosophila Brain

      • Nana Shimosako, Dafni Hadjieconomou, Iris Salecker
      Pages 57-69

    6. Immunofluorescent Labeling of Neural Stem Cells in the Drosophila Optic Lobe

      • Benjamin Perruchoud, Boris Egger
      Pages 71-78

    7. Using MARCM to Study Drosophila Brain Development

      • Gudrun Viktorin
      Pages 79-96

  4. Molluscs

    1. Front Matter

      Pages 115-115
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    2. Methods in Brain Development of Molluscs

      • Andreas Wanninger, Tim Wollesen
      Pages 117-125

  5. Xenopus Protocols

    1. Front Matter

      Pages 127-127
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    2. In Situ Hybridization and Immunostaining of Xenopus Brain

      • Kai-li Liu, Xiu-mei Wang, Zi-long Li, Rong-qiao He, Ying Liu
      Pages 129-141

    3. Microinjection Manipulations in the Elucidation of Xenopus Brain Development

      • Cristine Smoczer, Lara Hooker, Saqib S. Sachani, Michael J. Crawford
      Pages 143-154

    4. Morpholino Studies in Xenopus Brain Development

      • Jennifer E. Bestman, Hollis T. Cline
      Pages 155-171

  6. Zebrafish Protocols

    1. Front Matter

      Pages 173-173
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    2. Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification

      • Gilbert Lauter, Iris Söll, Giselbert Hauptmann
      Pages 175-185

    3. Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish

      • Adèle Faucherre, Hernán López-Schier
      Pages 187-195

    4. Anatomical Dissection of Zebrafish Brain Development

      • Katherine J. Turner, Thomas G. Bracewell, Thomas A. Hawkins
      Pages 197-214
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Keywords

About this book

Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes.  Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models.  Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.

Editors and Affiliations

  • Department of Biology, University of Fribourg, Fribourg, Switzerland

    Simon G Sprecher

Bibliographic Information

  • Book Title: Brain Development

  • Book Subtitle: Methods and Protocols

  • Editors: Simon G Sprecher

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-655-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-62703-654-2Published: 19 September 2013

  • Softcover ISBN: 978-1-4939-6025-5Published: 29 September 2016

  • eBook ISBN: 978-1-62703-655-9Published: 19 September 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 317

  • Number of Illustrations: 14 b/w illustrations, 49 illustrations in colour

  • Topics: Neurosciences, Neurobiology

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