- Reviews the latests developments in molecular biology techniqueds
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
-
In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology. Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.
- Table of contents (16 chapters)
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What Is Synthetic Biology?
Pages 3-6
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Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program
Pages 9-17
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BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit
Pages 19-30
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Overlap Extension PCR Cloning
Pages 31-42
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One-Step Isothermal Assembly of DNA Fragments
Pages 43-47
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Table of contents (16 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Synthetic Biology
- Editors
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- Karen M. Polizzi
- Cleo Kontoravdi
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1073
- Copyright
- 2013
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, New York
- eBook ISBN
- 978-1-62703-625-2
- DOI
- 10.1007/978-1-62703-625-2
- Hardcover ISBN
- 978-1-62703-624-5
- Softcover ISBN
- 978-1-4939-6286-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 226
- Number of Illustrations
- 32 b/w illustrations, 48 illustrations in colour
- Topics
