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  • © 2013

Synthetic Biology

  • Reviews the latests developments in molecular biology techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1073)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
  2. Introduction

    1. Front Matter

      Pages 1-1
    2. What Is Synthetic Biology?

      • Karen M. Polizzi
      Pages 3-6
  3. DNA Assembly

    1. Front Matter

      Pages 7-7
    2. Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program

      • Gang Li, Bing-Xue Dong, Yu-Huan Liu, Chang-Jie Li, Li-Ping Zhang
      Pages 9-17
    3. BioBrickâ„¢ Assembly Using the In-Fusion PCR Cloning Kit

      • Sean C. Sleight, Herbert M. Sauro
      Pages 19-30
    4. Overlap Extension PCR Cloning

      • Anton Bryksin, Ichiro Matsumura
      Pages 31-42
    5. One-Step Isothermal Assembly of DNA Fragments

      • Rui T. L. Rodrigues, Travis S. Bayer
      Pages 43-47
  4. Computational Tools for Modelling Biological Systems

    1. Front Matter

      Pages 175-175

About this book

 In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology.  Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.

Editors and Affiliations

  • Division of Molecular Biosciences, Imperial College London, London, United Kingdom

    Karen M. Polizzi

  • Department of Chemical Engineering, Imperial College London, London, United Kingdom

    Cleo Kontoravdi

Bibliographic Information

  • Book Title: Synthetic Biology

  • Editors: Karen M. Polizzi, Cleo Kontoravdi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-625-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2013

  • Hardcover ISBN: 978-1-62703-624-5Published: 01 September 2013

  • Softcover ISBN: 978-1-4939-6286-0Published: 23 August 2016

  • eBook ISBN: 978-1-62703-625-2Published: 31 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 226

  • Number of Illustrations: 32 b/w illustrations, 48 illustrations in colour

  • Topics: Biotechnology, Biochemical Engineering

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access