- Details methods that are used to study DNA using electrophoresis
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
-
In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.
- Table of contents (19 chapters)
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DNA Electrophoresis: Historical and Theoretical Perspectives
Pages 1-9
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Basic DNA Electrophoresis in Molecular Cloning: A Comprehensive Guide for Beginners
Pages 11-43
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Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-Dimensional (2D) Native–Native Agarose Gel Electrophoresis
Pages 45-61
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Analysis of DNA Structures from Eukaryotic Cells by Two-Dimensional Native–Native DNA Agarose Gel Electrophoresis
Pages 63-81
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Directionality of Replication Fork Movement Determined by Two-Dimensional Native–Native DNA Agarose Gel Electrophoresis
Pages 83-103
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Table of contents (19 chapters)
- Download Preface 1 PDF (24.9 KB)
- Download Sample pages 1 PDF (355.7 KB)
- Download Table of contents PDF (37.8 KB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- DNA Electrophoresis
- Book Subtitle
- Methods and Protocols
- Editors
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- Svetlana Makovets
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1054
- Copyright
- 2013
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, New York
- eBook ISBN
- 978-1-62703-565-1
- DOI
- 10.1007/978-1-62703-565-1
- Hardcover ISBN
- 978-1-62703-564-4
- Softcover ISBN
- 978-1-4939-5998-3
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 299
- Number of Illustrations
- 36 b/w illustrations, 20 illustrations in colour
- Topics
