Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1014)
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Table of contents (15 protocols)
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Front Matter
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Perspectives
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Front Matter
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Corneal Stem Cell Enrichment and Characterization
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Front Matter
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Corneal Cell Culture
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Front Matter
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Development of the Artificial Cornea
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Front Matter
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About this book
Continuous regeneration of the cornea is necessary to maintain this tissue in the transparent state that is essential for vision. Therapy for repair of the damaged anterior cornea is currently addressed through the transplantation of donor corneas or the delivery of limbal epithelial stem cells (LESC) to the ocular surface using amniotic membrane (AM) as a supporting scaffold. Research on the bioengineering of corneal equivalents as replacement tissue is underway to develop viable corneal prosthetics. Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine. Divided into four convenient sections, topics include the identification, characterisation and cultivation of LESC, as well as the investigation of biopolymers used as the basis for corneal substitutes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Corneal Regenerative Medicine: Methods and Protocols covers the fundamental techniques useful for both the laboratory and clinical settings. Â
Editors and Affiliations
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School of Pharmacy, University of Reading, Reading, United Kingdom
Bernice Wright, Che J Connon
Bibliographic Information
Book Title: Corneal Regenerative Medicine
Book Subtitle: Methods and Protocols
Editors: Bernice Wright, Che J Connon
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-432-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2013
Hardcover ISBN: 978-1-62703-431-9Published: 21 May 2013
Softcover ISBN: 978-1-4939-6272-3Published: 23 August 2016
eBook ISBN: 978-1-62703-432-6Published: 21 May 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 230
Number of Illustrations: 23 b/w illustrations, 33 illustrations in colour
Topics: Molecular Medicine, Ophthalmology