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  • © 2013

Dopamine

Methods and Protocols

Editors:

  • Focuses on leading, current approaches in the study of dopamine within a variety of biological systems
  • Features detailed protocols that have been tested in laboratories around the world
  • Includes expert tips and key implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 964)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. Biochemical, Proteomic, and Computational Tools

    1. Front Matter

      Pages 1-1
    2. Detection of Cell Surface Dopamine Receptors

      • Jiping Xiao, Clare Bergson
      Pages 3-13
    3. Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells

      • Peiying Yu, Van Anthony Villar, Pedro A. Jose
      Pages 15-24
    4. Methods of Dopamine Research in Retina Cells

      • Ana Lucia Marques Ventura, Fernando Garcia de Mello, Ricardo Augusto de Melo Reis
      Pages 25-42
    5. Modeling Spatial Aspects of Intracellular Dopamine Signaling

      • Kim T. Blackwell, Lane J. Wallace, BoHung Kim, Rodrigo F. Oliveira, Wonryull Koh
      Pages 61-75
  3. Cellular Imaging

    1. Front Matter

      Pages 77-77
    2. A Biophysical Approach for the Study of Dopamine Receptor Oligomerization

      • Sylwia Lukasiewicz, Agata Faron-Górecka, Marta Dziedzicka-Wasylewska
      Pages 79-94
    3. Detection of Receptor Heteromers Involving Dopamine Receptors by the Sequential BRET-FRET Technology

      • Gemma Navarro, Peter J. McCormick, Josefa Mallol, Carme Lluís, Rafael Franco, Antoni Cortés et al.
      Pages 95-105
    4. BRET Approaches to Characterize Dopamine and TAAR1 Receptor Pharmacology and Signaling

      • Stefano Espinoza, Bernard Masri, Ali Salahpour, Raul R. Gainetdinov
      Pages 107-122
    5. Dopaminergic Regulation of Dendritic Calcium: Fast Multisite Calcium Imaging

      • Wen-Liang Zhou, Katerina D. Oikonomou, Shaina M. Short, Srdjan D. Antic
      Pages 123-138
  4. Genetic Manipulation in Cells and Organisms

    1. Front Matter

      Pages 139-139
    2. Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish

      • Wendy Boehmler, Jessica Petko, Victor A. Canfield, Robert Levenson
      Pages 201-214
    3. Application of Cell-Specific Isolation to the Study of Dopamine Signaling in Drosophila

      • Eswar Prasad R. Iyer, Srividya Chandramouli Iyer, Daniel N. Cox
      Pages 215-225
  5. Electrochemical, Physiological, and Behavioral Analysis

    1. Front Matter

      Pages 227-227
    2. Regulation of Dopamine Transporter Expression by Neuronal Activity

      • Shalini Padmanabhan, Thach Pham, Balakrishna M. Prasad
      Pages 229-242

About this book

Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery.  In Dopamine: Methods and Protocols, experts and key figures within the field provide detailed protocols on leading approaches in the study of dopamine within biological systems. Divided into sections on cellular/biochemical, imaging, genetics, and electrophysiology, this collections includes protocols for bioluminescence and fluorescence imaging, receptor immunoprecipitation and proteomic analysis, creation and characterization of a mouse model of Parkinson’s disease, real time measurement of dopamine in the brain, and modeling signal transduction in silico.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and convenient, Dopamine: Methods and Protocols serves as an ideal guide for students and experts alike, as well as for anyone interested in exploring the vast and crucial field of dopamine research.

Reviews

From the book reviews:

“Designed for active researchers ranging from students to established scientists, the book is edited by a proteomics researcher working the in the area of dopamine and nicotine receptor protein interaction networks. … this book adds a collection of methods for contemporary techniques that should prove useful to researchers wishing to apply these approaches to their work.” (Beth Levant, Doody’s Book Reviews, May, 2013)

Editors and Affiliations

  • Krasnow Institute for Advanced Study, Dept. of Molecular Neuroscience, George Mason University, Fairfax, USA

    Nadine Kabbani

Bibliographic Information

  • Book Title: Dopamine

  • Book Subtitle: Methods and Protocols

  • Editors: Nadine Kabbani

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-251-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-250-6Published: 08 January 2013

  • Softcover ISBN: 978-1-4939-6333-1Published: 23 August 2016

  • eBook ISBN: 978-1-62703-251-3Published: 09 January 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 312

  • Topics: Neurosciences, Neurobiology, Neurochemistry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access