Methods in Molecular Biology

Nanoimaging

Methods and Protocols

Editors: Sousa, Alioscka A., Kruhlak, Michael J. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
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  • ISBN 978-1-62703-137-0
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Hardcover $199.00
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Softcover $199.00
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About this book

For more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology.  Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales.  Nanoimaging: Methods and Protocols presents a diverse collection of microscopy techniques and methodologies that provides guidance to successfully image cellular molecular complexes at nanometer spatial resolution.  The book's four parts cover: (1) light microscopy techniques with a special emphasis on methods that go beyond the classic diffraction-limited imaging; (2) electron microscopy techniques for high-resolution imaging of molecules, cells and tissues, in both two and three dimensions; (3) scanning probe microscopy techniques for imaging and probing macromolecular complexes and membrane surface topography;  and (4) complementary techniques on correlative microscopy, soft x-ray tomography and secondary ion mass spectrometry imaging.  Written in the successful format of the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Nanoimaging: Methods and Protocols highlights many of the most exciting possibilities in microscopy for the investigation of biological structures at the nano length molecular scales.

Reviews

From the reviews:

“The book is divided into 4 parts, each containing several chapters pertaining to a given field. … chapters are largely composed of detailed protocols describing a specific experimental procedure. Figures are mostly clear, and the tables and index are helpful. … I highly recommend this book to academic radiologists, imaging scientists, and molecular biologists.” (E. Edmund Kim, The Journal of Nuclear Medicine, Vol. 54 (5), May, 2013)

“The present volume … contains 28 protocols in diverse types of microscopy enabling scientists to obtain images of isolated molecular complexes, cells and tissues at nanoscale spatial resolution … . Each chapter has a standard structure … a clear style and useful illustrations. … this book should be read not only by very specialized scientists, but also by those who usually work with (very) classic microscopic methods, to open their minds and increase their appetite for more precise and complex measurements done on complex structures.” (Ioan I. Ardelean, Romanian Biochemical Journals, Vol. 50 (2), 2013)


Table of contents (26 chapters)

Table of contents (26 chapters)
  • Introduction: Nanoimaging Techniques in Biology

    Pages 1-10

    Sousa, Alioscka A. (et al.)

  • Live-Cell Imaging of Vesicle Trafficking and Divalent Metal Ions by Total Internal Reflection Fluorescence (TIRF) Microscopy

    Pages 13-26

    Loder, Merewyn K. (et al.)

  • 4Pi Microscopy

    Pages 27-41

    Schmidt, Roman (et al.)

  • Fluorescence In Situ Hybridization Applications for Super-Resolution 3D Structured Illumination Microscopy

    Pages 43-64

    Markaki, Yolanda (et al.)

  • Two-Color STED Imaging of Synapses in Living Brain Slices

    Pages 65-80

    Tønnesen, Jan (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-62703-137-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA in USD
  • ISBN 978-1-62703-136-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
price for USA in USD
  • ISBN 978-1-4939-5961-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Nanoimaging
Book Subtitle
Methods and Protocols
Editors
  • Alioscka A. Sousa
  • Michael J. Kruhlak
Series Title
Methods in Molecular Biology
Series Volume
950
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-137-0
DOI
10.1007/978-1-62703-137-0
Hardcover ISBN
978-1-62703-136-3
Softcover ISBN
978-1-4939-5961-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 510
Number of Illustrations
56 b/w illustrations, 76 illustrations in colour
Topics