Spermatogenesis
Methods and Protocols
Editors: Carrell, Douglas, Aston, Kenneth I. (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.
- Table of contents (40 chapters)
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Methods for Sperm Concentration Determination
Pages 3-12
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Methods of Sperm Vitality Assessment
Pages 13-19
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The Hypo-osmotic Swelling Test for Evaluation of Sperm Membrane Integrity
Pages 21-25
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Sperm Morphology Classification: A Rational Method for Schemes Adopted by the World Health Organization
Pages 27-37
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Sperm Morphology Assessment Using Strict (Tygerberg) Criteria
Pages 39-50
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Table of contents (40 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Spermatogenesis
- Book Subtitle
- Methods and Protocols
- Editors
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- Douglas Carrell
- Kenneth I. Aston
- Series Title
- Methods in Molecular Biology
- Series Volume
- 927
- Copyright
- 2013
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-62703-038-0
- DOI
- 10.1007/978-1-62703-038-0
- Hardcover ISBN
- 978-1-62703-037-3
- Softcover ISBN
- 978-1-4939-6267-9
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XVII, 554
- Topics
