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Methods in Molecular Biology

Diagnosis of Sexually Transmitted Diseases

Methods and Protocols

Editors: MacKenzie, Colin R., Henrich, Birgit (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-61779-937-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-61779-936-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-5885-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Sexually transmitted infections (STI) continue to be a major cause of morbidity and mortality, both in developed industrial countries as well as in the developing world. Human immunodeficiency virus infections and the ensuing opportunistic infections are a major drain on the human and financial resources of many countries in the developing world and even with the availability of effective treatment the epidemic is not yet contained. Screening in developing worlds is difficult and there is a need for simple reliable cheap diagnostic methods that can be performed at the point of care, on the same day and by staff with limited training. Molecular biological methods are very attractive for the diagnosis of STI since a well defined range of pathogens is responsible for the infection. Diagnosis of Sexually Transmitted Diseases: Methods and Protocols strives to cover the full range of molecular testing for STI. Chapters cover a variety of topics such as aspects of DNA extraction from small volume samples or difficult tissues, simple, nested or multiplex PCR, use of duplex primers or other modifications of primers and PCR conditions, sequence analysis for genotyping, denaturing gel analysis, microarrays using liquid beads or microspheres and silicon nanoparticle-enhanced microcantilever detection of DNA. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Diagnosis of Sexually Transmitted Diseases: Methods and Protocols will be a valuable reference for the scientist looking to establish molecular methodologies for the diagnosis of STI tests in their own lab.

Reviews

From the book reviews:

“This volume of Methods in Molecular Biology is the first to provide a comprehensive overview of molecular methods for sexually transmitted infections (STI). … This is a unique addition to the field of STI diagnostics and will be a useful reference for laboratory scientists wishing to incorporate the methods into their laboratories as well as for researchers and clinicians wishing to enhance their understanding of this area.” (Ameeta E. Singh, Doody’s Book Reviews, February, 2013)

Table of contents (29 chapters)

Table of contents (29 chapters)
  • Overview of Molecular Biological Methods for the Detection of Pathogens Causing Sexually Transmitted Infections

    Pages 1-20

    Vazquez, Fernando (et al.)

  • Guidelines for the Qualitative Detection of Viral Genomes in Dried Blood Spots

    Pages 21-34

    Gibellini, Davide (et al.)

  • Guidelines for the Quantification of HIV and HCV in Small Volume Whole Blood Samples

    Pages 35-50

    Bruns, Tony (et al.)

  • Guidelines for High-Resolution Genotyping of Chlamydia trachomatis Using Multilocus Sequence Analysis

    Pages 51-64

    Christerson, Linus (et al.)

  • Guidelines for the Use of Molecular Tests for the Detection and Genotyping of Human Papilloma Virus from Clinical Specimens

    Pages 65-101

    Lee, Sin Hang

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-61779-937-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-61779-936-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-5885-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Diagnosis of Sexually Transmitted Diseases
Book Subtitle
Methods and Protocols
Editors
  • Colin R. MacKenzie
  • Birgit Henrich
Series Title
Methods in Molecular Biology
Series Volume
903
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-61779-937-2
DOI
10.1007/978-1-61779-937-2
Hardcover ISBN
978-1-61779-936-5
Softcover ISBN
978-1-4939-5885-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 439
Topics