Methods in Molecular Biology

Receptor Binding Techniques

Editors: Davenport, Anthony P. (Ed.)

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  • Updates the previous edition with cutting-edge content
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand  to undergo a conformational change, leading to a physiological response or change in cell function.  A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of  Receptor Binding Techniques expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of  immunocytochemistry  for the cellular localization of receptor protein and hybridization to detect receptor mRNA.  Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and  green fluorescent protein to study  trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel ‘biased agonists’. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET).

Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Binding Techniques, Third Edition, aids scientists in continuing to study receptor binding.

Table of contents (13 chapters)

Table of contents (13 chapters)
  • Receptor Databases and Computational Websites for Ligand Binding

    Rana, Brinda K. (et al.)

    Pages 1-13

  • How to Use the IUPHAR Receptor Database to Navigate Pharmacological Data

    Mpamhanga, Chidochangu P. (et al.)

    Pages 15-29

  • Radioligand Binding Assays and Their Analysis

    Maguire, Janet J. (et al.)

    Pages 31-77

  • Use of Scintillation Proximity Assay to Measure Radioligand Binding to Immobilized Receptors Without Separation of Bound from Free Ligand

    Berry, Jenny (et al.)

    Pages 79-94

  • Visualization and Analysis of Vascular Receptors Using Confocal Laser Scanning Microscopy and Fluorescent Ligands

    Daly, Craig J. (et al.)

    Pages 95-107

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-61779-909-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-61779-908-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-5936-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Receptor Binding Techniques
Editors
  • Anthony P. Davenport
Series Title
Methods in Molecular Biology
Series Volume
897
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-909-9
DOI
10.1007/978-1-61779-909-9
Hardcover ISBN
978-1-61779-908-2
Softcover ISBN
978-1-4939-5936-5
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XII, 309
Topics