Methods in Molecular Biology

Antiviral Resistance in Plants

Methods and Protocols

Editors: Watson, John M., Wang, Ming-Bo (Eds.)

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  • Details many of the methods currently used to study RNA silencing in relation to viral infections in plants
  • Features step-by-step, easy to follow protocols from an international assortment of laboratories
  • Includes key tips and expert implementation advice to ensure successful results
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  • ISBN 978-1-61779-882-5
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About this book

Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants.  These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.

Table of contents (24 chapters)

Table of contents (24 chapters)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-61779-882-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover $179.99
price for USA in USD
Softcover $179.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Antiviral Resistance in Plants
Book Subtitle
Methods and Protocols
Editors
  • John M. Watson
  • Ming-Bo Wang
Series Title
Methods in Molecular Biology
Series Volume
894
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-882-5
DOI
10.1007/978-1-61779-882-5
Hardcover ISBN
978-1-61779-881-8
Softcover ISBN
978-1-4939-5722-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 380
Number of Illustrations
49 b/w illustrations, 10 illustrations in colour
Topics