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Methods in Molecular Biology

Sphingosine-1-Phosphate

Methods and Protocols

Editors: Pébay, Alice, Turksen, Kursad (Eds.)

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  • Features the important effects of S1P in cell development and physiopathology 
  • Includes detailed, tried-and-true protocols, ready for the lab
  • Contains expert tips and key troubleshooting advice to ensure successful results
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eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-61779-800-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-61779-799-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-5963-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells.  In Sphingosine-1-Phosphate: Methods and Protocols, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology.  As a volume in the Methods in Molecular Biology™ series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

 

Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols is a key resource for scientists working in this expanding and dynamic field.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate

    Pages 1-20

    Brizuela, Leyre (et al.)

  • Isoform-Selective Assays for Sphingosine Kinase Activity

    Pages 21-31

    Pitman, Melissa R. (et al.)

  • Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry

    Pages 33-44

    Bode, Constantin (et al.)

  • A Cleanup Method for Mass Spectrometry of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule

    Pages 45-54

    Morishige, Jun-ichi (et al.)

  • Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples

    Pages 55-67

    Visentin, Barbara (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-61779-800-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-61779-799-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-5963-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Sphingosine-1-Phosphate
Book Subtitle
Methods and Protocols
Editors
  • Alice Pébay
  • Kursad Turksen
Series Title
Methods in Molecular Biology
Series Volume
874
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-800-9
DOI
10.1007/978-1-61779-800-9
Hardcover ISBN
978-1-61779-799-6
Softcover ISBN
978-1-4939-5963-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 221
Number of Illustrations
29 b/w illustrations, 10 illustrations in colour
Topics