- Covers isolation characterization of numerous human embryonic stem cells as well as essential protocols in HES characterization
- Features detailed, cutting-edge methods and protocols
- Includes expert tips and key implementation advice for successful implementation
Buy this book
- About this book
-
Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult. In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology. Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
- Table of contents (20 chapters)
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Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology
Pages 1-12
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Human Embryonic Stem Cells Derived in Xeno-Free Conditions
Pages 13-32
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Procedures for Derivation and Characterisation of Human Embryonic Stem Cells from Odense, Denmark
Pages 33-51
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Principles for Derivation of Human Embryonic Stem Cells
Pages 53-68
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Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells
Pages 69-80
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Table of contents (20 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Human Embryonic Stem Cells Handbook
- Editors
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- Kursad Turksen
- Series Title
- Methods in Molecular Biology
- Series Volume
- 873
- Copyright
- 2012
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-61779-794-1
- DOI
- 10.1007/978-1-61779-794-1
- Hardcover ISBN
- 978-1-61779-793-4
- Softcover ISBN
- 978-1-4939-6262-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 313
- Number of Illustrations
- 36 b/w illustrations, 17 illustrations in colour
- Topics