Methods in Molecular Biology

Recombinant Protein Production in Yeast

Methods and Protocols

Editors: Bill, Roslyn M. (Ed.)

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  • Examines the process of preparation of expression vectors and disruption of yeast cells to enable to isolation of the recombinant protein
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-61779-770-5
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Hardcover $179.99
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Softcover $149.00
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  • ISBN 978-1-4939-5871-9
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About this book

In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Optimising Yeast as a Host for Recombinant Protein Production (Review)

    Pages 1-9

    Bonander, Nicklas (et al.)

  • Which Yeast Species Shall I Choose? Saccharomyces cerevisiae Versus Pichia pastoris (Review)

    Pages 11-23

    Darby, Richard A. J. (et al.)

  • Preparation of Pichia pastoris Expression Plasmids

    Pages 25-40

    Logez, Christel (et al.)

  • Preparation of Saccharomyces cerevisiae Expression Plasmids

    Pages 41-46

    Drew, David (et al.)

  • Codon Optimisation for Heterologous Gene Expression in Yeast

    Pages 47-55

    Hedfalk, Kristina

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-61779-770-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-61779-769-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-5871-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Recombinant Protein Production in Yeast
Book Subtitle
Methods and Protocols
Editors
  • Roslyn M. Bill
Series Title
Methods in Molecular Biology
Series Volume
866
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+business Media, LLC
eBook ISBN
978-1-61779-770-5
DOI
10.1007/978-1-61779-770-5
Hardcover ISBN
978-1-61779-769-9
Softcover ISBN
978-1-4939-5871-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 248
Number of Illustrations
41 b/w illustrations, 6 illustrations in colour
Topics