Exon Skipping
Methods and Protocols
Editors: Aartsma-Rus, Annemieke (Ed.)
- Provides an overview of the exon skipping field
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
-
“Next generation” sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal models requiring a more extensive set of protocols. Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate “real polymorphisms” from mutations that affect splicing. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping.
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- Download Table of contents PDF (110.8 KB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Exon Skipping
- Book Subtitle
- Methods and Protocols
- Editors
-
- Annemieke Aartsma-Rus
- Series Title
- Methods in Molecular Biology
- Series Volume
- 867
- Copyright
- 2012
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media,LLC
- eBook ISBN
- 978-1-61779-767-5
- DOI
- 10.1007/978-1-61779-767-5
- Hardcover ISBN
- 978-1-61779-766-8
- Softcover ISBN
- 978-1-4939-6206-8
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 440
- Number of Illustrations
- 62 b/w illustrations, 8 illustrations in colour
- Topics
