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Lipases and Phospholipases

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Georgina Sandoval

    1. CIATEJ-CONACYT, Industrial Biotechnology Unit, Guadalajara, Mexico

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  • Details fundamental issues and new applications of lipases and phospholipases
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 861)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
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  2. Overview of Lipases, Phospholipases and Related Enzymes

    1. Front Matter

      Pages 1-1
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  3. OVERVIEW OF LIPASES, PHOSPHOLIPASES AND RELATED ENZYMES

    1. Lipases: An Overview

      • Leticia Casas-Godoy, Sophie Duquesne, Florence Bordes, Georgina Sandoval, Alain Marty
      Pages 3-30

    2. Lipases or Esterases: Does It Really Matter? Toward a New Bio-Physico-Chemical Classification

      • Yassine Ben Ali, Robert Verger, Abdelkarim Abousalham
      Pages 31-51

    3. Study of New Feruloyl Esterases to Understand Lipase Evolution: The Case of Bacillus flexus

      • Mónica Sánchez-González, Allan Blanco-Gámez, Roberto Parra-Saldívar, Juan Carlos Mateos-Díaz, María Isabel Estrada-Alvarado
      Pages 53-61

    4. Phospholipases: An Overview

      • Ahmed Aloulou, Yassine Ben Ali, Sofiane Bezzine, Youssef Gargouri, Michael H. Gelb
      Pages 63-85

  4. Screening and Sources of Lipases, Phospholipases and Its Inhibitors

    1. Front Matter

      Pages 87-87
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  5. SCREENING AND SOURCES OF LIPASES, PHOSPHOLIPASES AND ITS INHIBITORS

    1. High-Throughput Screening Method for Lipases/Esterases

      • Eduardo Mateos-Díaz, Jorge Alberto Rodríguez, María de los Ángeles Camacho-Ruiz, Juan Carlos Mateos-Díaz
      Pages 89-100

    2. Functional-Based Screening Methods for Lipases, Esterases, and Phospholipases in Metagenomic Libraries

      • Dolores Reyes-Duarte, Manuel Ferrer, Humberto García-Arellano
      Pages 101-113

    3. Plant Lipases: Partial Purification of Carica papaya Lipase

      • Ivanna Rivera, Juan Carlos Mateos-Díaz, Georgina Sandoval
      Pages 115-122

    4. Plant Phospholipases: An Overview

      • Geliang Wang, Stephen Ryu, Xuemin Wang
      Pages 123-137

    5. Lipase and Phospholipase Inhibitors: Design and Applications

      • Mark E. Venable
      Pages 139-148

    6. High-Throughput Assay of Secreted Phospholipases A2 Inhibitors

      • Wallace Thompson, Rob C. Oslund, James Bollinger, Heather Ewing, Michael H. Gelb
      Pages 149-158

  6. Cloning, Production, Purification and Characterization of Lipases and Phospholipases

    1. Front Matter

      Pages 159-159
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  7. CLONING, PRODUCTION, PURIFICATION AND CHARACTERIZATION OF LIPASES AND PHOSPHOLIPASES

    1. Heterologous Expression Systems for Lipases: A Review

      • Francisco Valero
      Pages 161-178

    2. Streptomyces Phospholipase D Cloning and Production

      • Yozo Nakazawa
      Pages 179-200

    3. Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses

      • Julian Gomez-Cambronero, Karen M. Henkels
      Pages 201-225

    4. Lipases Production by Solid-State Fermentation: The Case of Rhizopus homothallicus in Perlite

      • Susana Velasco-Lozano, Tania Volke-Sepulveda, Ernesto Favela-Torres
      Pages 227-237

    5. Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27

      • Pablo Fuciños, Roberto González, Estrella Atanes, Ana Belén Fernández Sestelo, Nelson Pérez-Guerra, Lorenzo Pastrana et al.
      Pages 239-266

    6. Purification, Biochemical and Kinetic Properties of Recombinant Staphylococcus aureus Lipase

      • Habib Horchani, Ahmed Fendri, Hanen Louati, Adel Sayari, Youssef Gargouri, Robert Verger
      Pages 267-282
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Keywords

About this book

Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes.

Editors and Affiliations

  • CIATEJ-CONACYT, Industrial Biotechnology Unit, Guadalajara, Mexico

    Georgina Sandoval

Bibliographic Information

  • Book Title: Lipases and Phospholipases

  • Book Subtitle: Methods and Protocols

  • Editors: Georgina Sandoval

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-600-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-599-2Published: 17 March 2012

  • Softcover ISBN: 978-1-4939-5877-1Published: 23 August 2016

  • eBook ISBN: 978-1-61779-600-5Published: 17 March 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 547

  • Number of Illustrations: 83 b/w illustrations, 18 illustrations in colour

  • Topics: Biochemistry, general, Enzymology

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