Methods in Molecular Biology

Plant Fungal Pathogens

Methods and Protocols

Editors: Bolton, Melvin D., Thomma, Bart P.H.J. (Eds.)

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  • Highlights the latest techniques critical for the study of molecular plant pathology
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-61779-501-5
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Softcover $199.00
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About this book

Over the course of evolution, fungi have adapted to occupy specific niches, from symbiotically inhabiting the flora of the intestinal tract of mammals to saprophytic growth on leaf litter resting on the forest floor.  In Plant Fungal Pathogens: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study fungal plant pathogens. These include methods and techniques for model systems such as Arabidopsis thaliana as well as crop plants, aspects of fungal biology, genome annotation, next-generation sequencing, and fungal transformation and molecular tools for disease and/or pathogen quantification that are critical for revealing the role for a fungal gene of interest in disease development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Plant Fungal Pathogens: Methods and Protocols seeks to aid scientists in the further study in current techniques that cover a wide-range of methods to study molecular aspects of pathogenesis.

Reviews

From the reviews:

“The authors provide the latest information on topics including methods in molecular biology and highlighting some of the latest techniques critical for students pursuing molecular plant pathology. … All the chapters are well illustrated with charts and graphs and with necessary photographs. … should be available in school libraries experienced fungal research laboratories and university libraries. This is specially aimed at researchers new to the molecular biology field.” (Asha Janadaree Dissanayake and Kevin D. Hyde, Fungal Diversity, Vol. 59, March, 2013)

“The current volume is a unique collection of 41 chapters from a diverse group of scientists, researchers, and academics working on different aspects of the molecular basis of fungal pathogens and pathogenesis. … This volume will be useful for both students and researchers in the fields of botany and forestry, agriculture, mycology, microbiology, plant disease and diagnostics, molecular plant pathology, horticulture science, biomolecular sciences, life sciences, fungal molecular biology, fungal biotechnology, microbial biotechnology, and food technology.” (S. K. Basu, Plant Science Bulletin, Vol. 59 (1), 2013)

Table of contents (41 chapters)

Table of contents (41 chapters)
  • Fungal ABC Transporter Deletion and Localization Analysis

    Pages 1-16

    Kovalchuk, Andriy (et al.)

  • Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens- Mediated Transformation

    Pages 17-45

    Frandsen, Rasmus John Normand (et al.)

  • Activity-Based Protein Profiling of Infected Plants

    Pages 47-59

    Kaschani, Farnusch (et al.)

  • The Use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves

    Pages 61-74

    Ma, Lisong (et al.)

  • Proteomic Techniques for Plant–Fungal Interactions

    Pages 75-96

    Vincent, Delphine (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-61779-501-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
Softcover $199.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Plant Fungal Pathogens
Book Subtitle
Methods and Protocols
Editors
  • Melvin D. Bolton
  • Bart P.H.J. Thomma
Series Title
Methods in Molecular Biology
Series Volume
835
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
Distribution Rights
Distribution rights for India: Mehul Book Sales, Mumbai, India
eBook ISBN
978-1-61779-501-5
DOI
10.1007/978-1-61779-501-5
Hardcover ISBN
978-1-61779-500-8
Softcover ISBN
978-1-4939-5898-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 648
Topics