Methods in Molecular Biology

Chromatin Remodeling

Methods and Protocols

Editors: Morse, Randall H. (Ed.)

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  • Features a wide range of methods, including in vitro, in vivo, visual, molecular, genomic, and computational
  • Authored by recognized leaders in the field of chromatin biology
  • Includes expert tips and implementation advice in order to ensure successful results
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eBook $139.00
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  • ISBN 978-1-61779-477-3
  • Digitally watermarked, DRM-free
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Hardcover $179.00
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Softcover $179.00
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About this book

Chromatin is of central importance to gene regulation in eukaryotes.  Reflecting this singular role for chromatin, numerous approaches have evolved in the laboratory over the past three decades to study chromatin structure and its alterations. Methods of investigating chromatin remodeling, whether in changes in nucleosome structure or position with respect to the incorporated DNA or in histone modifications, have progressed rapidly over the recent years.  In Chromatin Remodeling: Methods and Protocols, expert researchers contribute chapters which include methods for investigating chromatin remodeling in vitro and in vivo, in yeast, plants, and mammalian cells, and at local and global levels.  Both gene-specific and genome-wide approaches are covered, and in recognition of the increasing prevalence of the latter type of study, the final two chapters focus on bioinformatic/computational approaches to analyzing genome-wide data on chromatin structure. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and essential, Chromatin Remodeling: Methods and Protocols serves as a key source of clear techniques as well as a collection of chapters that can inspire future techniques in this vital field of study.

Reviews

From the reviews:

“This is an outstanding and timely collection of methods concerning how one can investigate chromatin remodelling. … There are detailed laboratory procedures and notes attached to all chapters, and this enable readers to use the volume as a laboratory manual. … The editor, Randall Morse, has to be congratulated on producing such an excellent book; a book that I recommend most highly to researchers in this arena.” (Raymond Waters, Human Genetics, Vol. 131, 2012)

Table of contents (27 chapters)

Table of contents (27 chapters)
  • Strain Construction and Screening Methods for a Yeast Histone H3/H4 Mutant Library

    Pages 1-14

    Dai, Junbiao (et al.)

  • Measuring Dynamic Changes in Histone Modifications and Nucleosome Density during Activated Transcription in Budding Yeast

    Pages 15-27

    Govind, Chhabi K. (et al.)

  • Monitoring the Effects of Chromatin Remodelers on Long-Range Interactions In Vivo

    Pages 29-45

    Kiefer, Christine M. (et al.)

  • Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease

    Pages 47-61

    Bryant, Gene O.

  • Analysis of Nucleosome Positioning Using a Nucleosome-Scanning Assay

    Pages 63-87

    Infante, Juan Jose (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-61779-477-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $179.00
price for USA in USD
Softcover $179.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Chromatin Remodeling
Book Subtitle
Methods and Protocols
Editors
  • Randall H. Morse
Series Title
Methods in Molecular Biology
Series Volume
833
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-477-3
DOI
10.1007/978-1-61779-477-3
Hardcover ISBN
978-1-61779-476-6
Softcover ISBN
978-1-4939-6213-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 456
Topics