Methods in Molecular Biology

DNA Electrophoresis Protocols for Forensic Genetics

Editors: Alonso, Antonio (Ed.)

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  • |Offers a comprehensive coverage of the most modern current electrophoretic protocols and interpretation guidelines used in forensic genetic.
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
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  • ISBN 978-1-61779-461-2
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Hardcover $179.00
price for USA in USD
  • ISBN 978-1-61779-460-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-6219-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of  PCR amplicons obtained from DNA genomic markers with different  inheritance patterns. In DNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It  includes protocols for  profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and  mtDNA  hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful  Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Reviews

From the reviews:

“The purpose is to offer a comprehensive book on the most modern current electrophoretic protocols and interpretation guidelines used in forensic genetics for the analysis of amplified human DNA fragments and DNA sequencing. … researchers, forensic experts, and others interested in the field of DNA electrophoresis protocols will find this book of immense help. … This is a unique resource that students, researchers, and forensic experts will find both interesting and helpful.” (Omer Iqbal, Doody’s Book Reviews, May, 2012)

Table of contents (26 chapters)

Table of contents (26 chapters)
  • An Overview of DNA Typing Methods for Human Identification: Past, Present, and Future

    Pages 3-16

    Thompson, Robyn (et al.)

  • Capillary Electrophoresis and 5-Channel LIF Detection of a 26plex Autosomal STR Assay for Human Identification

    Pages 17-29

    Hill, Carolyn R.

  • Capillary Electrophoresis of MiniSTR Markers to Genotype Highly Degraded DNA Samples

    Pages 31-42

    Coble, Michael D.

  • Interpretation Guidelines of a Standard Y-chromosome STR 17-plex PCR-CE Assay for Crime Casework

    Pages 43-56

    Roewer, Lutz (et al.)

  • Capillary Electrophoresis of an X-Chromosome STR Decaplex for Kinship Deficiency Cases

    Pages 57-71

    Gusmão, Leonor (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-61779-461-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-61779-460-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-6219-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
DNA Electrophoresis Protocols for Forensic Genetics
Editors
  • Antonio Alonso
Series Title
Methods in Molecular Biology
Series Volume
830
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-461-2
DOI
10.1007/978-1-61779-461-2
Hardcover ISBN
978-1-61779-460-5
Softcover ISBN
978-1-4939-6219-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 394
Topics