Methods in Molecular Biology

Two Hybrid Technologies

Methods and Protocols

Editors: Suter, Bernhard, Wanker, Erich E. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-61779-455-1
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About this book

Protein–protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping. Divided into two convenient sections, the first gives a survey of protocols that are currently employed for Y2H high-throughput screens by different expert labs in the field. Rather than detailing the principles of screening, which have been described previously, the focus is on different implementations of Y2H interactome mapping. The second section of the book considers innovative PPI detection methods that have the potential to emerge as alternative high-throughput methodologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Two Hybrid Technologies: Methods and Protocols  supplies researchers with a comprehensive toolbox for the identification of biologically relevant protein interactions.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Matrix-Based Yeast Two-Hybrid Screen Strategies and Comparison of Systems

    Pages 1-20

    Häuser, Roman (et al.)

  • Array-Based Yeast Two-Hybrid Screens: A Practical Guide

    Pages 21-38

    Häuser, Roman (et al.)

  • High-Throughput Yeast Two-Hybrid Screening

    Pages 39-61

    Roberts, George G., III (et al.)

  • A Stringent Yeast Two-Hybrid Matrix Screening Approach for Protein–Protein Interaction Discovery

    Pages 63-87

    Worseck, Josephine M. (et al.)

  • High-Throughput Yeast Two-Hybrid Screening of Complex cDNA Libraries

    Pages 89-102

    Mohr, Kerstin (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-61779-455-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $219.99
price for USA in USD
Softcover $179.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Two Hybrid Technologies
Book Subtitle
Methods and Protocols
Editors
  • Bernhard Suter
  • Erich E. Wanker
Series Title
Methods in Molecular Biology
Series Volume
812
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-455-1
DOI
10.1007/978-1-61779-455-1
Hardcover ISBN
978-1-61779-454-4
Softcover ISBN
978-1-4939-5811-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 329
Topics