Methods in Molecular Biology

Amino Acid Analysis

Methods and Protocols

Editors: Alterman, Michail A., Hunziker, Peter (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-61779-445-2
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Hardcover $179.00
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Softcover $179.00
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About this book

Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology.  Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

Table of contents (28 chapters)

  • Rapid LC–MS/MS Profiling of Protein Amino Acids and Metabolically Related Compounds for Large-Scale Assessment of Metabolic Phenotypes

    Gu, Liping (et al.)

    Pages 1-11

  • Combination of an AccQ·Tag-Ultra Performance Liquid Chromatographic Method with Tandem Mass Spectrometry for the Analysis of Amino Acids

    Salazar, Carolina (et al.)

    Pages 13-28

  • Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry for Quantitative Amino Acid Analysis

    Bunk, David M. (et al.)

    Pages 29-38

  • Analysis of Underivatized Amino Acids: Zwitterionic Hydrophilic Interaction Chromatography Combined with Triple Quadrupole Tandem Mass Spectrometry

    Dell’mour, Madeleine (et al.)

    Pages 39-46

  • Amino Acid Analysis via LC–MS Method After Derivatization with Quaternary Phosphonium

    Inagaki, Shinsuke (et al.)

    Pages 47-54

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-61779-445-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-61779-444-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-5889-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Amino Acid Analysis
Book Subtitle
Methods and Protocols
Editors
  • Michail A. Alterman
  • Peter Hunziker
Series Title
Methods in Molecular Biology
Series Volume
828
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-445-2
DOI
10.1007/978-1-61779-445-2
Hardcover ISBN
978-1-61779-444-5
Softcover ISBN
978-1-4939-5889-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 363
Topics