Methods in Molecular Biology

Rho GTPases

Methods and Protocols

Editors: Rivero, Francisco (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-61779-442-1
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Hardcover $179.00
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Softcover $179.00
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About this book

Although initially described as major regulators of cytoskeletal remodeling, Rho GTPases have been implicated in the establishment of polarity, endocytosis, vesicle trafficking, morphogenesis, cytokinesis, transcriptional activation, cell cycle progression, and apoptosis, to mention a few. In addition, Rho GTPases have acquired medical relevance because of their participation in tumorigenesis and metastasis, in cardiovascular conditions, and as targets of infectious agents. The field has broadened even more with the contribution of studies in model organisms (plants, amoebas, fungi, invertebrates), each adding a particular view to the complexity of the family and vastly enriching our perception of these important signaling components. Divided into five convenient sections, Rho GTPase: Methods and Protocols provides an historical overview of the field and an account of the phylogenetics of the Rho family, general biochemical methods, and functional assays that allow monitoring the consequences of manipulating Rho GTPases in a variety of contexts. Additionally, the volume devotes a section to advanced imaging methods and to recently developed high throughput methods, closing with techniques specifically designed for studies in selected non-mammalian model organisms. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Rho GTPase: Methods and Protocols provides techniques that are standard for researchers in the field but also includes protocols for those who, being already familiar with some of the techniques, wish to explore additional aspects.

Table of contents (26 chapters)

  • Historical Overview of Rho GTPases

    Ridley, Anne J.

    Pages 3-12

  • Rho GTPases: Deciphering the Evolutionary History of a Complex Protein Family

    Eliáš, Marek (et al.)

    Pages 13-34

  • Biochemical Assays to Characterize Rho GTPases

    Jaiswal, Mamta (et al.)

    Pages 37-58

  • How to Analyze Bacterial Toxins Targeting Rho GTPases

    Bielek, Heike (et al.)

    Pages 59-75

  • Assessing Ubiquitylation of Rho GTPases in Mammalian Cells

    Doye, Anne (et al.)

    Pages 77-86

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-61779-442-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-61779-441-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-5896-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Rho GTPases
Book Subtitle
Methods and Protocols
Editors
  • Francisco Rivero
Series Title
Methods in Molecular Biology
Series Volume
827
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-442-1
DOI
10.1007/978-1-61779-442-1
Hardcover ISBN
978-1-61779-441-4
Softcover ISBN
978-1-4939-5896-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 407
Topics