Protein Expression in Mammalian Cells
Methods and Protocols
Editors: Hartley, James L (Ed.)
Free Preview- Explores techniques to express proteins in mammalian cells in order to relate the research most closely to human biology
- Features step-by-step methods and protocols, ready for the lab
- Includes expert tips and implementation advice to ensure successful results
Buy this book
- About this book
-
Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins. - Reviews
-
From the reviews:
“This book is really a great help for all those that wish to enter the field and I am sure even for those who are already involved with recombinant protein production in mammalian cells. … the book is intended as well for basic researchers aimed to study native or recombinant proteins to highlight … protein misfolding or the role of proteins in cancer and in general is intended for all those scientists aiming to contribute to solve the many mysteries still connoting the protein world.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 56, 2012)
- Table of contents (16 chapters)
-
-
Why Proteins in Mammalian Cells?
Pages 1-12
-
Large-Scale Transfection of Mammalian Cells
Pages 13-26
-
Selection of High Expressing Mammalian Cells by Surface Display of Reporters
Pages 27-39
-
Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles
Pages 41-63
-
Transfection of Difficult-to-Transfect Primary Mammalian Cells
Pages 65-74
-
Table of contents (16 chapters)
Recommended for you
Bibliographic Information
- Bibliographic Information
-
- Book Title
- Protein Expression in Mammalian Cells
- Book Subtitle
- Methods and Protocols
- Editors
-
- James L Hartley
- Series Title
- Methods in Molecular Biology
- Series Volume
- 801
- Copyright
- 2012
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-61779-352-3
- DOI
- 10.1007/978-1-61779-352-3
- Hardcover ISBN
- 978-1-61779-351-6
- Softcover ISBN
- 978-1-4939-6160-3
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 276
- Topics
