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Chemical Genomics and Proteomics

Reviews and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Edward D. Zanders

    1. Royston, United Kingdom

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  • Focuses on the foremost, current technologies being used in the fields of chemical genomics and proteomics
  • Includes clear and concise laboratory protocols, ready for use
  • Features expert tips and implementation advice to ensure successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 800)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Reviews

    1. Front Matter

      Pages 1-1
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    2. Overview of Chemical Genomics and Proteomics

      • Edward D. Zanders
      Pages 3-10

    3. Exploring Chemical Space: Recent Advances in Chemistry

      • Yung-Sing Wong
      Pages 11-23

    4. In Silico Design of Small Molecules

      • Paul H. Bernardo, Joo Chuan Tong
      Pages 25-31

    5. Surface Plasmon Resonance for Proteomics

      • Nico J. de Mol
      Pages 33-53

  3. Protocols

    1. Front Matter

      Pages 55-55
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    2. Biomimetic Affinity Ligands for Immunoglobulins Based on the Multicomponent Ugi Reaction

      • Graziella El Khoury, Laura A. Rowe, Christopher R. Lowe
      Pages 57-74

    3. Preparation of Photo-Cross-Linked Small Molecule Affinity Matrices for Affinity Selection of Protein Targets for Biologically Active Small Molecules

      • Hiroshi Takayama, Takashi Moriya, Naoki Kanoh
      Pages 75-83

    4. Profiling Cellular Myristoylation and Palmitoylation Using ω-Alkynyl Fatty Acids

      • Rami N. Hannoush
      Pages 85-94

    5. Fluorescence Labels in Kinases: A High-Throughput Kinase Binding Assay for the Identification of DFG-Out Binding Ligands

      • Jeffrey R. Simard, Daniel Rauh
      Pages 95-117

    6. Electrochemical Aptamer Sensor for Small Molecule Assays

      • Xin Liu, Wang Li, Xiahong Xu, Jiang Zhou, Zhou Nie
      Pages 119-132

    7. Miniaturized, Microarray-Based Assays for Chemical Proteomic Studies of Protein Function

      • Jonathan M. Blackburn, Aubrey Shoko, Natasha Beeton-Kempen
      Pages 133-162

    8. Glycan Microarrays

      • Xuezheng Song, Jamie Heimburg-Molinaro, David F. Smith, Richard D. Cummings
      Pages 163-171

    9. Resolving Bottlenecks for Recombinant Protein Expression in E. coli

      • Yoav Peleg, Tamar Unger
      Pages 173-186

    10. Recombinant Protein Expression in the Baculovirus-Infected Insect Cell System

      • Tamar Unger, Yoav Peleg
      Pages 187-199

    11. Systems for the Cell-Free Synthesis of Proteins

      • Lei Kai, Christian Roos, Stefan Haberstock, Davide Proverbio, Yi Ma, Friederike Junge et al.
      Pages 201-225

    12. Protein Identification by MALDI-TOF Mass Spectrometry

      • Judith Webster, David Oxley
      Pages 227-240

  4. Back Matter

    Pages 241-243
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Keywords

About this book

Chemical genomics technology has been steadily improving, delivering new biological probes and drugs, and the explicit use of the term ‘chemical proteomics’ has increased with it, as proteins have always been at the heart of this technology.  In Chemical Genomics and Proteomics: Reviews and Protocols, experts in the field present updated reviews of the chemistry of small molecules and their interaction with protein targets as well as detailed protocols that cover different types of ligands, carbohydrates, and lipids.  For example, the generation of their protein targets and methods for measuring their interactions is covered.  Written in the highly successful Methods in Molecular Biology™ series format, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Thorough and up to date,  Chemical Genomics and Proteomics: Reviews and Protocols aims to provide inspiration to those who wish to use chemical genomics and proteomics in their work and develop this young field into full maturity through the incorporation of the new biological and chemical technologies beginning to emerge here.

Editors and Affiliations

  • Royston, United Kingdom

    Edward D. Zanders

Bibliographic Information

  • Book Title: Chemical Genomics and Proteomics

  • Book Subtitle: Reviews and Protocols

  • Editors: Edward D. Zanders

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-349-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-348-6Published: 01 October 2011

  • Softcover ISBN: 978-1-4939-6226-6Published: 23 August 2016

  • eBook ISBN: 978-1-61779-349-3Published: 02 October 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 243

  • Number of Illustrations: 71 b/w illustrations

  • Topics: Biochemistry, general, Proteomics, Gene Function

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