Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 797)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (15 protocols)
-
Front Matter
-
Back Matter
About this book
Since the first report of an engineered oncolytic virus, there has been a continuing and steady increase of interest in the field, and while bench research remains vital for the translation of research in this field, its success depends on breakthroughs in clinical studies. Oncolytic Viruses: Methods and Protocols describes the construction and purification of capsid-modified adenoviruses as well as oncolytic adenoviruses, presents protocols for many individual virus species including engineering and preparation of oncolytic HSV, propagation, purification, and in vivo testing of oncolytic VSV, details properties of oncolytic reovirus and NDV, and describes the generation and testing of next generation of oncolytic vaccinia virus. As the host immune system plays a critical role in determining efficacy of oncolytic viruses, two chapters are devoted to the study of immune response. Recent advances in stem cell research have led the field in two distinct directions: the use of stem cells as carrier vehicles for oncolytic viruses and the targeting of cancer stem cells. As such, the volume describes the use of explant tissue samples from patients to potentially provide useful information predicting responses prior to clinical translation. Written in the successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Â
Authoritative and easily accessible, Oncolytic Viruses: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential and vital field.
Editors and Affiliations
-
Jennerex Biotherapeutics, Inc., San Francisco, USA
David H. Kirn
-
School of Medicine, Dept. Pathology & Immunology, Washington University, St.Louis, USA
Ta-Chiang Liu
-
Hillman Cancer Center, University of Pittsburgh, Pittsburgh, USA
Stephen H. Thorne
Bibliographic Information
Book Title: Oncolytic Viruses
Book Subtitle: Methods and Protocols
Editors: David H. Kirn, Ta-Chiang Liu, Stephen H. Thorne
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-340-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-339-4Published: 27 September 2011
Softcover ISBN: 978-1-4939-5812-2Published: 23 August 2016
eBook ISBN: 978-1-61779-340-0Published: 25 September 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 241
Number of Illustrations: 24 b/w illustrations, 11 illustrations in colour
Topics: Infectious Diseases