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Methods in Molecular Biology

Oncolytic Viruses

Methods and Protocols

Editors: Kirn, David H., Liu, Ta-Chiang, Thorne, Stephen H. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-61779-340-0
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Hardcover $149.99
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Softcover $149.00
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About this book

Since the first report of an engineered oncolytic virus, there has been a continuing and steady increase of interest in the field, and while bench research remains vital for the translation of research in this field, its success depends on breakthroughs in clinical studies. Oncolytic Viruses: Methods and Protocols describes the construction and purification of capsid-modified adenoviruses as well as oncolytic adenoviruses, presents protocols for many individual virus species including engineering and preparation of oncolytic HSV, propagation, purification, and in vivo testing of oncolytic VSV, details properties of oncolytic reovirus and NDV, and describes the generation and testing of next generation of oncolytic vaccinia virus. As the host immune system plays a critical role in determining efficacy of oncolytic viruses, two chapters are devoted to the study of immune response. Recent advances in stem cell research have led the field in two distinct directions: the use of stem cells as carrier vehicles for oncolytic viruses and the targeting of cancer stem cells. As such, the volume describes the use of explant tissue samples from patients to potentially provide useful information predicting responses prior to clinical translation. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Oncolytic Viruses: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential and vital field.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Oncolytic Herpes Simplex Virus Engineering and Preparation

    Pages 1-19

    Agarwalla, Pankaj K. (et al.)

  • Construction of Capsid-Modified Adenoviruses by Recombination in Yeast and Purification by Iodixanol-Gradient

    Pages 21-34

    Giménez-Alejandre, Marta (et al.)

  • Construction of Targeted and Armed Oncolytic Adenoviruses

    Pages 35-52

    Doronin, Konstantin (et al.)

  • Syrian Hamster Tumor Model to Study Oncolytic Ad5-Based Vectors

    Pages 53-63

    Dhar, Debanjan (et al.)

  • Adenoviral Gene Expression and Replication in Human Tumor Explant Models

    Pages 65-78

    Halldén, Gunnel

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-61779-340-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
Softcover $149.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Oncolytic Viruses
Book Subtitle
Methods and Protocols
Editors
  • David H. Kirn
  • Ta-Chiang Liu
  • Stephen H. Thorne
Series Title
Methods in Molecular Biology
Series Volume
797
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-340-0
DOI
10.1007/978-1-61779-340-0
Hardcover ISBN
978-1-61779-339-4
Softcover ISBN
978-1-4939-5812-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 241
Number of Illustrations
24 b/w illustrations, 11 illustrations in colour
Topics