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Methods in Molecular Biology

Single Molecule Enzymology

Methods and Protocols

Editors: Mashanov, Gregory I., Batters, Christopher (Eds.)

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  • Serves as an ideal guide for the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail
  • Features cutting-edge, lab ready protocols
  • Includes key tips and expert implementation advice to ensure successful results
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-61779-261-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-61779-260-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-6190-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy.  In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Single Enzyme Studies: A Historical Perspective

    Pages 1-9

    Knight, Alex E.

  • Functional Surface Attachment in a Sandwich Geometry of GFP-Labeled Motor Proteins

    Pages 11-18

    Bormuth, Volker (et al.)

  • Studying Kinesin’s Enzymatic Cycle Using a Single-Motor Confocal Motility Assay, Employing Förster Resonance Energy Transfer

    Pages 19-32

    Lansky, Zdenek (et al.)

  • Fluorescence Imaging with One Nanometer Accuracy: In Vitro and In Vivo Studies of Molecular Motors

    Pages 33-56

    Hoffman, Melinda Tonks (et al.)

  • Snapshots of Kinesin Motors on Microtubule Tracks

    Pages 57-70

    Fourniol, Franck J. (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-61779-261-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-61779-260-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-6190-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Single Molecule Enzymology
Book Subtitle
Methods and Protocols
Editors
  • Gregory I. Mashanov
  • Christopher Batters
Series Title
Methods in Molecular Biology
Series Volume
778
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-261-8
DOI
10.1007/978-1-61779-261-8
Hardcover ISBN
978-1-61779-260-1
Softcover ISBN
978-1-4939-6190-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 294
Topics