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Methods in Molecular Biology

Vertebrate Embryogenesis

Embryological, Cellular, and Genetic Methods

Editors: Pelegri, Francisco (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-61779-210-6
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Hardcover $189.00
price for USA in USD
  • ISBN 978-1-61779-209-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA in USD
  • ISBN 978-1-4939-6161-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

One of the striking findings of modern developmental biology has been the high degree of conservation of signaling and developmental mechanisms amongst different animal species. Such conservation allows information learned from a given organism to be applicable to other species, including humans, and has validated the use of a few model systems to deduce general biological principles. In spite of this underlying conservation, however, each species has unique characteristics arising from its evolutionary history. Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods attempts to address the increasingly important need of straddling species boundaries in the context of a single research program by compiling research protocols used in a wide range of vertebrate species. In fact, this volume has been designed so that readers can readily find information on species other than the one with which they may be most familiar. These protocols include not only embryological methods, but also cellular and genetic approaches that have complemented and expanded our understanding of embryonic development.  In addition, a number of chapters highlight a specific method that is in principle applicable to multiple species, such as TILLING and ZFN-mediated mutagenesis, the generation of Embryonic Stem (ES) cell lines, and nuclear/oocyte transfer.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods serves as an ideal guide to the molecular, cell, and developmental biology community and will hopefully contribute to the ongoing collective effort towards a better understanding of the beauty and logic of vertebrate development.

Reviews

From the reviews:

“Prof. Francisco J. Pelegrini … deserves the great merit of assembling a twenty-four chapters volume that will surely help researchers and educators thanks to both the choice of the topics covered (which includes even a chapter dealing with the laboratory guidelines for animal care) and to the easy-to-follow protocols that highlight each chapter.” (CarloAlberto Redi, European Journal of Histochemistry, Vol. 56, 2012)

Table of contents (25 chapters)

  • Approaches and Species in the History of Vertebrate Embryology

    Hopwood, Nick

    Pages 1-20

  • Manipulating and Imaging the Early Xenopus laevis Embryo

    Danilchik, Michael V.

    Pages 21-54

  • Manipulation of Gene Function in Xenopus laevis

    Mimoto, Mizuho S. (et al.)

    Pages 55-75

  • Developmental Genetics in Xenopus tropicalis

    Geach, Timothy J. (et al.)

    Pages 77-117

  • Embryological and Genetic Manipulation of Chick Development

    Gammill, Laura S. (et al.)

    Pages 119-137

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-61779-210-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA in USD
  • ISBN 978-1-61779-209-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA in USD
  • ISBN 978-1-4939-6161-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Vertebrate Embryogenesis
Book Subtitle
Embryological, Cellular, and Genetic Methods
Editors
  • Francisco Pelegri
Series Title
Methods in Molecular Biology
Series Volume
770
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-210-6
DOI
10.1007/978-1-61779-210-6
Hardcover ISBN
978-1-61779-209-0
Softcover ISBN
978-1-4939-6161-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 605
Topics