Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 767)
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Table of contents (33 protocols)
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Front Matter
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Derivation
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Front Matter
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Growth, Maintenance, and Expansion
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Front Matter
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Growth, Maintenance and Expansion
Keywords
About this book
Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.
Reviews
From the reviews:
“The volume is a compilation of 33 detailed protocols divided into six parts … . All the protocols are superbly illustrated with many figures and charts presented in full colours. I think this book is a must for all those are working with human pluripotent stem cells, no matter if students entering the field or colleagues already involved, each of them will get the chance to sharpen their conceptual insights.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 56, 2012)
Editors and Affiliations
Bibliographic Information
Book Title: Human Pluripotent Stem Cells
Book Subtitle: Methods and Protocols
Editors: Philip H. Schwartz, Robin L. Wesselschmidt
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-201-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-200-7Published: 09 August 2011
Softcover ISBN: 978-1-4939-5802-3Published: 23 August 2016
eBook ISBN: 978-1-61779-201-4Published: 09 August 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XX, 468
Topics: Cell Biology, Stem Cells, Cell Culture