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Methods in Molecular Biology

Strain Engineering

Methods and Protocols

Editors: Williams, James A. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
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  • ISBN 978-1-61779-197-0
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Softcover $169.00
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About this book

Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, E. coli-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast Saccharomyces cerevisiae as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Strain Engineering: Methods and Protocols serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering.

Table of contents (29 chapters)

Table of contents (29 chapters)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-61779-197-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover $219.99
price for USA in USD
Softcover $169.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Strain Engineering
Book Subtitle
Methods and Protocols
Editors
  • James A. Williams
Series Title
Methods in Molecular Biology
Series Volume
765
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-197-0
DOI
10.1007/978-1-61779-197-0
Hardcover ISBN
978-1-61779-196-3
Softcover ISBN
978-1-4939-5846-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 480
Number of Illustrations
81 b/w illustrations, 2 illustrations in colour
Topics