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Nitrogen Fixation

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Markus W. Ribbe

    1. , Dept Molecular Biology & Biochemistry, University of California, Irvine, Irvine, USA

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  • Compiles a detailed collection of techniques to study one of the most difficult problems in protein biochemistry
  • Features practical, step-by-step methodologies
  • Includes key tips and expert implementation advice to ensure successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 766)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
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  2. Nitrogen Fixation

    1. Front Matter

      Pages 1-1
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    2. Historic Overview of Nitrogenase Research

      • Yilin Hu, Markus W. Ribbe
      Pages 3-7

    3. Mechanism of Mo-Dependent Nitrogenase

      • Zhi-Yong Yang, Karamatullah Danyal, Lance C. Seefeldt
      Pages 9-29

    4. Assembly of Nitrogenase MoFe Protein

      • Chi-Chung Lee, Aaron W. Fay, Jared A. Wiig, Markus W. Ribbe, Yilin Hu
      Pages 31-47

    5. Genomic Analysis of Nitrogen Fixation

      • Ina P. O’Carroll, Patricia C. Dos Santos
      Pages 49-65

    6. Enzymatic Systems with Homology to Nitrogenase

      • Jürgen Moser, Markus J. Bröcker
      Pages 67-77

  3. Genetic and Biochemical Methods

    1. Front Matter

      Pages 79-79
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    2. Molecular Biology and Genetic Engineering in Nitrogen Fixation

      • Patricia C. Dos Santos
      Pages 81-92

    3. Purification of Nitrogenase Proteins

      • Jared A. Wiig, Chi-Chung Lee, Aaron W. Fay, Yilin Hu, Markus W. Ribbe
      Pages 93-103

    4. Assays of Nitrogenase Reaction Products

      • William E. Newton, Michael J. Dilworth
      Pages 105-127

    5. Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase

      • Jürgen Moser, Markus J. Bröcker
      Pages 129-143

  4. Spectroscopic Methods

    1. Front Matter

      Pages 145-145
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    2. X-Ray Crystallography

      • Lauren E. Roth, F. Akif Tezcan
      Pages 147-164

    3. X-Ray Absorption Spectroscopy

      • Serena DeBeer
      Pages 165-176

    4. Small Angle X-Ray Scattering Spectroscopy

      • David W. Mulder, John W. Peters
      Pages 177-189

    5. Electron Paramagnetic Resonance Spectroscopy

      • Karamatullah Danyal, Zhi-Yong Yang, Lance C. Seefeldt
      Pages 191-205

    6. Magnetic Circular Dichroism Spectroscopy

      • Brian J. Hales
      Pages 207-219

    7. Mössbauer Spectroscopy

      • Boi Hanh Huynh
      Pages 221-235

  5. Chemical Methods

    1. Front Matter

      Pages 237-237
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Keywords

About this book

Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia.  In Nitrogen Fixation: Methods and Protocols, recognized experts in the field provide an up-to-date, in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, ranging from genetic, biochemical, spectroscopic, and chemical methods to theoretical calculations. In addition, techniques used to study an enzyme system that is homologous to nitrogenase are described in this book.  Written in the highly successful Methods in Molecular Biology™ series format, methods chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and cutting-edge, Nitrogen Fixation: Methods and Protocols will be useful for anyone interested in nitrogenase research and willing to venture further toward addressing the remaining mechanistic and biosynthetic questions of this fascinating enzyme system.

Editors and Affiliations

  • , Dept Molecular Biology & Biochemistry, University of California, Irvine, Irvine, USA

    Markus W. Ribbe

Bibliographic Information

  • Book Title: Nitrogen Fixation

  • Book Subtitle: Methods and Protocols

  • Editors: Markus W. Ribbe

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-194-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-193-2Published: 17 August 2011

  • Softcover ISBN: 978-1-4939-5841-2Published: 23 August 2016

  • eBook ISBN: 978-1-61779-194-9Published: 17 August 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 326

  • Number of Illustrations: 72 b/w illustrations

  • Topics: Biochemistry, general, Proteomics, Enzymology

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