Therapeutic Oligonucleotides
Methods and Protocols
Editors: Goodchild, John (Ed.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.
- Reviews
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From the reviews:
“The book is written for both professionals and novices interested in the field of oligonucleotide therapeutics. It also would be appropriate for graduate students and research scientists interested in this area. … This is an extremely useful resource that provides different ways that oligonucleotides may be applied as therapeutics. Very few books cover the field as thoroughly and as clearly as this one.” (Omer Iqbal, Doody’s Book Reviews, November, 2011)
- Table of contents (21 chapters)
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Therapeutic Oligonucleotides
Pages 1-15
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Dinucleotides Containing 3′-S-Phosphorothiolate Linkages
Pages 17-30
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2′-O,4′-C-Methyleneoxymethylene Bridged Nucleic Acids (2′,4′-BNACOC)
Pages 31-57
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A Non-covalent Peptide-Based Strategy for Ex Vivo and In Vivo Oligonucleotide Delivery
Pages 59-73
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Cell-Penetrating Peptides-Based Strategies for the Delivery of Splice Redirecting Antisense Oligonucleotides
Pages 75-89
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Table of contents (21 chapters)
- Download Preface 1 PDF (59.5 KB)
- Download Sample pages 1 PDF (179.8 KB)
- Download Table of contents PDF (51.7 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Therapeutic Oligonucleotides
- Book Subtitle
- Methods and Protocols
- Editors
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- John Goodchild
- Series Title
- Methods in Molecular Biology
- Series Volume
- 764
- Copyright
- 2011
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-61779-188-8
- DOI
- 10.1007/978-1-61779-188-8
- Hardcover ISBN
- 978-1-61779-187-1
- Softcover ISBN
- 978-1-4939-5822-1
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 340
- Number of Illustrations
- 70 b/w illustrations
- Topics
