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Methods in Molecular Biology

Cell Cycle Synchronization

Methods and Protocols

Editors: Banfalvi, Gaspar (Ed.)

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  • Offers practical lab protocols for synchronizing mammalian, plant, yeast, fungal, and even bacterial cells
  • Serves a wide variety of scientists, from cell biologists to medical students to pharmacologists
  • Features key tips and expert implementation advice to ensure successful results
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Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-61779-182-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-61779-181-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-6194-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe.  In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events.  Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Detailed and easy to follow,  Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Overview of Cell Synchronization

    Pages 1-23

    Banfalvi, Gaspar

  • Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation

    Pages 25-45

    Banfalvi, Gaspar

  • Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells

    Pages 47-63

    Castedo, Maria (et al.)

  • Large-Scale Mitotic Cell Synchronization

    Pages 65-74

    Dulla, Kalyan (et al.)

  • Synchronization of Mammalian Cell Cultures by Serum Deprivation

    Pages 75-83

    Langan, Thomas J. (et al.)

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-61779-182-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-61779-181-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-6194-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cell Cycle Synchronization
Book Subtitle
Methods and Protocols
Editors
  • Gaspar Banfalvi
Series Title
Methods in Molecular Biology
Series Volume
761
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-182-6
DOI
10.1007/978-1-61779-182-6
Hardcover ISBN
978-1-61779-181-9
Softcover ISBN
978-1-4939-6194-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 295
Topics