Cell Cycle Synchronization
Methods and Protocols
Editors: Banfalvi, Gaspar (Ed.)
Free Preview- Offers practical lab protocols for synchronizing mammalian, plant, yeast, fungal, and even bacterial cells
- Serves a wide variety of scientists, from cell biologists to medical students to pharmacologists
- Features key tips and expert implementation advice to ensure successful results
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- About this book
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To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
- Table of contents (19 chapters)
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Overview of Cell Synchronization
Pages 1-23
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Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation
Pages 25-45
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Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells
Pages 47-63
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Large-Scale Mitotic Cell Synchronization
Pages 65-74
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Synchronization of Mammalian Cell Cultures by Serum Deprivation
Pages 75-83
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Table of contents (19 chapters)
- Download Preface 1 PDF (1.4 MB)
- Download Sample pages 1 PDF (3.6 MB)
- Download Table of contents PDF (1.3 MB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Cell Cycle Synchronization
- Book Subtitle
- Methods and Protocols
- Editors
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- Gaspar Banfalvi
- Series Title
- Methods in Molecular Biology
- Series Volume
- 761
- Copyright
- 2011
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-61779-182-6
- DOI
- 10.1007/978-1-61779-182-6
- Hardcover ISBN
- 978-1-61779-181-9
- Softcover ISBN
- 978-1-4939-6194-8
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 295
- Topics