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Methods in Molecular Biology

Gel-Free Proteomics

Methods and Protocols

Editors: Gevaert, Kris, Vandekerckhove, Joël (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-61779-148-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
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  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-61779-147-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-5819-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. Gel-Free Proteomics: Methods and Protocols addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five chapters, this detailed volume meticulously describes vital procedures needed to perform gel-free proteomics, ranging from sample preparation, isotope labeling for differential proteomics, enrichment technologies for modified proteins and peptides, and bioinformatics. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Gel-Free Proteomics: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this critical field.

Table of contents (25 chapters)

Table of contents (25 chapters)
  • Mass Spectrometry-Driven Proteomics: An Introduction

    Pages 1-27

    Helsens, Kenny (et al.)

  • Metabolic Labeling of Model Organisms Using Heavy Nitrogen (15N)

    Pages 29-42

    Gouw, Joost W. (et al.)

  • Trypsin-Catalyzed Oxygen-18 Labeling for Quantitative Proteomics

    Pages 43-54

    Qian, Wei-Jun (et al.)

  • ICPL Labeling Strategies for Proteome Research

    Pages 55-64

    Lottspeich, Friedrich (et al.)

  • Quantitative Proteome Analysis Using Isobaric Peptide Termini Labeling (IPTL)

    Pages 65-76

    Arntzen, Magnus Ø. (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-61779-148-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-61779-147-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-5819-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Gel-Free Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Kris Gevaert
  • Joël Vandekerckhove
Series Title
Methods in Molecular Biology
Series Volume
753
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-148-2
DOI
10.1007/978-1-61779-148-2
Hardcover ISBN
978-1-61779-147-5
Softcover ISBN
978-1-4939-5819-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 400
Topics