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Methods in Molecular Biology

DNA Recombination

Methods and Protocols

Editors: Tsubouchi, Hideo (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
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  • ISBN 978-1-61779-129-1
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Hardcover $219.99
price for USA in USD
  • ISBN 978-1-61779-128-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
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  • ISBN 978-1-4939-5778-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Homologous recombination is important in various aspects of DNA metabolism, including damage repair, replication, telomere maintenance, and meiosis, and yeast genetics has successfully provided a framework for the mechanism of homologous recombination. Divided into four convenient sections, DNA Recombination: Methods and Protocols covers recent techniques that best utilize the advantages of the yeast system, prescribing to the belief that yeast will keep serving as a great model organism to study homologous recombination. Chapters have also been included for such exceptions as the group of genes involved in recombination that are found solely in higher eukaryotes, such as BRCA2. And looking forward, a necessary step in the direction of understanding the homologous recombination process is to isolate the machine and let it work in a test tube.  Understanding the design by studying the appearance and behavior of the machinery as a single molecule will be an important milestone toward understanding the mechanism of action of the machinery. Techniques covering these topics have also been included.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, DNA Recombination: Methods and Protocols serves as an ideal guide to scientists of all backgrounds with its well-honed methodologies and strives to bring the reader to the next level of understanding regarding this vital subject.

Table of contents (32 chapters)

Table of contents (32 chapters)
  • Methods to Study Mitotic Homologous Recombination and Genome Stability

    Pages 3-13

    Zheng, Xiuzhong (et al.)

  • Characterizing Resection at Random and Unique Chromosome Double-Strand Breaks and Telomere Ends

    Pages 15-31

    Ma, Wenjian (et al.)

  • Characterization of Meiotic Recombination Initiation Sites Using Pulsed-Field Gel Electrophoresis

    Pages 33-45

    Farmer, Sarah (et al.)

  • Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using Single-Stranded DNA

    Pages 47-63

    Blitzblau, Hannah G. (et al.)

  • Detection of Covalent DNA-Bound Spo11 and Topoisomerase Complexes

    Pages 65-77

    Hartsuiker, Edgar

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-61779-129-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-61779-128-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
price for USA in USD
  • ISBN 978-1-4939-5778-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
DNA Recombination
Book Subtitle
Methods and Protocols
Editors
  • Hideo Tsubouchi
Series Title
Methods in Molecular Biology
Series Volume
745
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-129-1
DOI
10.1007/978-1-61779-129-1
Hardcover ISBN
978-1-61779-128-4
Softcover ISBN
978-1-4939-5778-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 565
Topics