Mammalian Cell Viability
Methods and Protocols
Editors: Stoddart, Martin J. (Ed.)
Free Preview- Features protocols utilizing both common lab equipment and specialized, cutting-edge equipment
- Facilitates the multiplexing of methods for the greatest levels of success
- Contains expert tips and key implementation advice
Buy this book
- About this book
-
Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.
Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.
- Table of contents (21 chapters)
-
-
Cell Viability Assays: Introduction
Pages 1-6
-
Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods
Pages 7-12
-
Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay
Pages 13-19
-
WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells
Pages 21-25
-
Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye
Pages 27-32
-
Table of contents (21 chapters)
Recommended for you
Bibliographic Information
- Bibliographic Information
-
- Book Title
- Mammalian Cell Viability
- Book Subtitle
- Methods and Protocols
- Editors
-
- Martin J. Stoddart
- Series Title
- Methods in Molecular Biology
- Series Volume
- 740
- Copyright
- 2011
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-61779-108-6
- DOI
- 10.1007/978-1-61779-108-6
- Hardcover ISBN
- 978-1-61779-107-9
- Softcover ISBN
- 978-1-4939-6153-5
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 240
- Topics
