Methods in Molecular Biology

Mammalian Chromosome Engineering

Methods and Protocols

Editors: Hadlaczky, Gyula (Ed.)

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  • Provides a precious resource for scientists interested in new ideas in gene technology
  • Features practical protocols from the cutting-edge of this fast-paced field
  • Includes expert tips and key implementation advice crucial for getting optimal results
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  • ISBN 978-1-61779-099-7
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Hardcover $149.99
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About this book

The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry.  Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system.  Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Developing Extrachromosomal Gene Expression Vector Technologies: An Overview

    Pages 1-17

    Wade-Martins, Richard

  • High Capacity Extrachromosomal Gene Expression Vectors

    Pages 19-40

    Hibbitt, Olivia (et al.)

  • Naturally Occurring Minichromosome Platforms in Chromosome Engineering: An Overview

    Pages 41-56

    Raimondi, Elena

  • Chromosome Transfer Via Cell Fusion

    Pages 57-67

    Paulis, Marianna

  • Insertional Engineering of Chromosomes with Sleeping Beauty Transposition: An Overview

    Pages 69-85

    Grabundzija, Ivana (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-61779-099-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-61779-098-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-5775-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Mammalian Chromosome Engineering
Book Subtitle
Methods and Protocols
Editors
  • Gyula Hadlaczky
Series Title
Methods in Molecular Biology
Series Volume
738
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-099-7
DOI
10.1007/978-1-61779-099-7
Hardcover ISBN
978-1-61779-098-0
Softcover ISBN
978-1-4939-5775-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
IX, 257
Topics