Methods in Molecular Biology

Telomeres and Telomerase

Methods and Protocols

Editors: Songyang, Zhou (Ed.)

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  • Features cutting-edge protocols from this expanding and celebrated field of study
  • Details biochemical, molecular, and proteomic approaches
  • Contains expert tips and key implementation advice
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eBook $109.00
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  • ISBN 978-1-61779-092-8
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Hardcover $179.99
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Softcover $149.00
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About this book

New and rapid advances in technology have equipped us with a variety of tools and platforms to ask fundamental questions of telomere regulation and have allowed investigators to carry out experiments using diverse model systems.  For example, proteomic, genomic, and molecular approaches have afforded us unprecedented insight into the complex protein interaction networks at work on the telomere chromatin and the detailed information regarding telomere dynamics in response to stress or stimuli.  Telomeres and Telomerase: Methods and Protocols, Second Edition builds upon the telomerase assays featured in the popular first edition to encompass many different assays that allow investigators to query the function of telomere proteins and the responses of the telomere DNA, including detailed examinations of biochemical, molecular, and proteomic approaches.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Telomeres and Telomerase: Methods and Protocols, Second Edition serves as an ideal, up-to-date guide for investigators further pursing this vital field of study.

Reviews

From the reviews of the second edition:

“The book provides different assays to study the function of telomere proteins and the responses of the telomere DNA. It also provides protocols for biochemical, molecular, and proteomic approaches in the understanding of telomere dynamics. It appears to be intended for investigators interested in the field of telomere and telomerase, but it also will be of immense help to other clinicians interested in cell growth, proliferation, aging, and cancer.” (Omer Iqbal, Doody’s Book Reviews, February, 2012)

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Introduction to Telomeres and Telomerase

    Pages 1-11

    Songyang, Zhou

  • Analysis of Average Telomere Length in Cultured Human Cells

    Pages 13-19

    Liu, Dan

  • Telomere Length Analysis by Quantitative Fluorescent In situ Hybridization (Q-FISH)

    Pages 21-31

    Ourliac-Garnier, Isabelle (et al.)

  • Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH)

    Pages 33-46

    Ourliac-Garnier, Isabelle (et al.)

  • Telomere G-Overhang Length Measurement Method 1: The DSN Method

    Pages 47-54

    Zhao, Yong (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-61779-092-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $179.99
price for USA in USD
Softcover $149.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Telomeres and Telomerase
Book Subtitle
Methods and Protocols
Editors
  • Zhou Songyang
Series Title
Methods in Molecular Biology
Series Volume
735
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-092-8
DOI
10.1007/978-1-61779-092-8
Hardcover ISBN
978-1-61779-091-1
Softcover ISBN
978-1-4939-5627-2
Series ISSN
1064-3745
Edition Number
2
Number of Pages
X, 191
Topics