Methods in Molecular Biology

High-Throughput Next Generation Sequencing

Methods and Applications

Editors: Kwon, Young Min, Ricke, Steven C. (Eds.)

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  • Provides techniques necessary to grasp system-wide landscapes of complex molecular events
  • Supplies practical chapters with easy to follow instructions
  • Features key tips and implementation advice from the experts
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eBook $139.00
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  • ISBN 978-1-61779-089-8
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Hardcover $219.99
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  • ISBN 978-1-61779-088-1
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  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
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  • ISBN 978-1-4939-6164-1
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About this book

Due to their novel concepts and extraordinary high-throughput sequencing capacity, the “next generation sequencing” methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities. These methods are now being recognized as essential tools for a more comprehensive and deeper understanding of the mechanisms underlying many biological processes.  In High-Throughput Next Generation Sequencing: Methods and Applications, experts in the field explore the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities; however, the methods described in this book will also offer general applications relevant to the study of any living organisms.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, High-Throughput Next Generation Sequencing: Methods and Applications is an excellent collection of chapters to aid all scientists who wish to apply these innovative research tools to enhance their own pursuits in microbiology and also biology in general.

Reviews

From the reviews:

“This well-illustrated book details the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities, in addition to general applications relevant to the study of any living organism. … The book is intended for scientists interested in this area, but even graduate students and other researchers field may find this book useful.” (Omer Iqbal, Doody’s Book Reviews, March, 2012)

“There are definitely some highlights that will make this book of use to both NGS Newbies and experienced practitioners. … many readers will find this a very useful book.” (Michael A. Quail, Human Genetics, Vol. 130, 2011)

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Helicos Single-Molecule Sequencing of Bacterial Genomes

    Steinmann, Kathleen E. (et al.)

    Pages 3-24

  • Whole-Genome Sequencing of Unculturable Bacterium Using Whole-Genome Amplification

    Hongoh, Yuichi (et al.)

    Pages 25-33

  • RNA Sequencing and Quantitation Using the Helicos Genetic Analysis System

    Raz, Tal (et al.)

    Pages 37-49

  • Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing

    Ozsolak, Fatih (et al.)

    Pages 51-61

  • Discovery of Bacterial sRNAs by High-Throughput Sequencing

    Liu, Jane M. (et al.)

    Pages 63-79

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-61779-089-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-61779-088-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-6164-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
High-Throughput Next Generation Sequencing
Book Subtitle
Methods and Applications
Editors
  • Young Min Kwon
  • Steven C. Ricke
Series Title
Methods in Molecular Biology
Series Volume
733
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-089-8
DOI
10.1007/978-1-61779-089-8
Hardcover ISBN
978-1-61779-088-1
Softcover ISBN
978-1-4939-6164-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 308
Topics