Editors:
- Provides techniques necessary to grasp system-wide landscapes of complex molecular events
- Supplies practical chapters with easy to follow instructions
- Features key tips and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 733)
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Table of contents (20 protocols)
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Front Matter
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Gene Expression Analysis
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Front Matter
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Microbial Diversity
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Front Matter
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Sequence Profiling for Functional Analysis
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Front Matter
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About this book
Due to their novel concepts and extraordinary high-throughput sequencing capacity, the “next generation sequencing” methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities. These methods are now being recognized as essential tools for a more comprehensive and deeper understanding of the mechanisms underlying many biological processes. In High-Throughput Next Generation Sequencing: Methods and Applications, experts in the field explore the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities; however, the methods described in this book will also offer general applications relevant to the study of any living organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, High-Throughput Next Generation Sequencing: Methods and Applications is an excellent collection of chapters to aid all scientists who wish to apply these innovative research tools to enhance their own pursuits in microbiology and also biology in general.
Reviews
From the reviews:
“This well-illustrated book details the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities, in addition to general applications relevant to the study of any living organism. … The book is intended for scientists interested in this area, but even graduate students and other researchers field may find this book useful.” (Omer Iqbal, Doody’s Book Reviews, March, 2012)
“There are definitely some highlights that will make this book of use to both NGS Newbies and experienced practitioners. … many readers will find this a very useful book.” (Michael A. Quail, Human Genetics, Vol. 130, 2011)Editors and Affiliations
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, Department of Poultry Science, University of Arkansas, Fayetteville, USA
Young Min Kwon
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Department of Food Science, University of Arkansas, Fayetteville, USA
Steven C. Ricke
Bibliographic Information
Book Title: High-Throughput Next Generation Sequencing
Book Subtitle: Methods and Applications
Editors: Young Min Kwon, Steven C. Ricke
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-089-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-088-1Published: 22 March 2011
Softcover ISBN: 978-1-4939-6164-1Published: 23 August 2016
eBook ISBN: 978-1-61779-089-8Published: 23 March 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 308
Topics: Human Genetics, Microbial Genetics and Genomics, Gene Expression