Overview
- Includes well-established and tested methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains well-organized themes and key implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 724)
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Table of contents (19 protocols)
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Front Matter
Keywords
About this book
Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.
Reviews
From the reviews:
“This text can be considered an introductory compendium of current methods and protocols in Molecular Biology for the use of Fixed Paraffin-Embedded Tissues that will be particularly suited to new researchers in the field who are eager to develop expertise. It is an easily readable and technically comprehensive book that would be ideal for initial experimental planning which can be complemented by other bibliographic sources and scientific journals for in-depth discussions of particular techniques and their potential applications.” (Flavia Troglio and Manuela Vecchi, Molecular Biotechnology, 2012)
Editors and Affiliations
Bibliographic Information
Book Title: Formalin-Fixed Paraffin-Embedded Tissues
Book Subtitle: Methods and Protocols
Editors: Fahd Al-Mulla
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-055-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-054-6Published: 16 February 2011
Softcover ISBN: 978-1-4939-6171-9Published: 23 August 2016
eBook ISBN: 978-1-61779-055-3Published: 03 March 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 312
Topics: Molecular Medicine, Pathology