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Methods in Molecular Biology

Argonaute Proteins

Methods and Protocols

Editors: Hobman, Tom C., Duchaine, Thomas F. (Eds.)

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  • Helps researchers study these versatile proteins in a wide variety of cell types
  • Features practical, step-by-step protocols which open the door to future progress in the field of molecular therapeutics
  • Contains expert tips and vital implementation advice to ensure success
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eBook $139.00
price for USA in USD
  • ISBN 978-1-61779-046-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
Softcover $179.00
price for USA in USD
About this book

With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner.  Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels.  In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.

Reviews

From the reviews:

“It can be fully appreciated the high quality of the book, the so well organized and sequentially logic presentation of the chapters. … giving a very update and detailed presentation of the entire Argonaute family members, their evolutionary conservation in Eukaryotes, mechanisms of  action, methods to study  in vitro and in vivo … . each biologist and physician, either involved in research or in clinical application will find irreplaceable this book. Hope the reader will get his own copy of this exciting book.” (Manuela Monti, European Journal of Histochemistry, July, 2011)

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Purification of Native Argonaute Complexes from the Fission Yeast Schizosaccharomyces pombe

    Pages 1-13

    Buker, Shane M. (et al.)

  • Chromatin Immunoprecipitation in Fission Yeast

    Pages 15-28

    Volpe, Thomas A. (et al.)

  • Biochemical Analyzes of Endogenous Argonaute Complexes Immunopurified with Anti-Argonaute Monoclonal Antibodies

    Pages 29-43

    Miyoshi, Keita (et al.)

  • Mapping of Ago2–GW182 Functional Interactions

    Pages 45-62

    Yao, Bing (et al.)

  • Continuous Density Gradients to Study Argonaute and GW182 Complexes Associated with the Endocytic Pathway

    Pages 63-76

    Gibbings, Derrick

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-61779-046-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
Softcover $179.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Argonaute Proteins
Book Subtitle
Methods and Protocols
Editors
  • Tom C. Hobman
  • Thomas F. Duchaine
Series Title
Methods in Molecular Biology
Series Volume
725
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-046-1
DOI
10.1007/978-1-61779-046-1
Hardcover ISBN
978-1-61779-045-4
Softcover ISBN
978-1-4939-5827-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 344
Topics