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Polyamines

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Anthony E. Pegg

    1. College of Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Robert A. Casero, Jr.

    1. School of Medicine, Sidney Kimmel Comprehensive, Johns Hopkins University, Baltimore, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Contains comprehensive and extensive practical coverage of polyamine-related methodologies in a wide variety of subject areas
  • Features step-by-step protocols, clearly written and readily reproducible
  • Includes expert tips and key implementation advice to ensure methodological success
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 720)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Current Status of the Polyamine Research Field

      • Anthony E. Pegg, Robert A. Casero Jr.
      Pages 3-35

  3. Identification of Polyamine Metabolic Enzymes and Characterization of Polyamine-Regulated Genes and Ion Channels

    1. Front Matter

      Pages 37-37
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    2. Exploring Polyamine Biosynthetic Diversity Through Comparative and Functional Genomics

      • Anthony J. Michael
      Pages 39-50

    3. Characterization of Genes for Polyamine Modulon

      • Kazuei Igarashi, Keiko Kashiwagi
      Pages 51-65

    4. Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines

      • Lan Xiao, Jian-Ying Wang
      Pages 67-79

    5. Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extreme Thermophiles

      • Tairo Oshima, Toshiyuki Moriya, Yusuke Terui
      Pages 81-111

    6. Polyamine Block of Inwardly Rectifying Potassium Channels

      • Harley T. Kurata, Wayland W. L. Cheng, Colin G. Nichols
      Pages 113-126

  4. Transgenic Models for Study of Polyamine Function

    1. Front Matter

      Pages 127-127
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    2. Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism

      • David J. Feith
      Pages 129-141

    3. Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Lipid Metabolism

      • Anne Uimari, Mervi T. Hyvönen, Eija Pirinen, Leena Alhonen
      Pages 143-158

    4. Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine

      • Xiaojing Wang, Anthony E. Pegg
      Pages 159-170

  5. Enzymes Involved in Polyamine Catabolism and Posttranslational Protein Modification

    1. Front Matter

      Pages 171-171
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  6. Enzymes Involved in Polyamine Catabolism and Post-Translational Protein Modification

    1. A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Response and Disease

      • Andrew C. Goodwin, Tracy R. Murray-Stewart, Robert A. Casero Jr.
      Pages 173-181

    2. Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases

      • Panagiotis N. Moschou, Kalliopi A. Roubelakis-Angelakis
      Pages 183-194

    3. Assay of Deoxyhypusine Synthase Activity

      • Edith C. Wolff, Seung Bum Lee, Myung Hee Park
      Pages 195-205

    4. Assay of Deoxyhypusine Hydroxylase Activity

      • Jong Hwan Park, Edith C. Wolff, Myung Hee Park
      Pages 207-216

  7. Regulation of Polyamine Content

    1. Front Matter

      Pages 217-217
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    2. Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase

      • Erin K. Willert, Lisa N. Kinch, Margaret A. Phillips
      Pages 219-235
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Keywords

About this book

Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development.  In Polyamines: Methods and Protocols, leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study.  The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Polyamines: Methods and Protocols provides a key resource for all scientists pursuing the study of this dynamic and significant aspect of cellular biology.

Editors and Affiliations

  • College of Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey, USA

    Anthony E. Pegg

  • School of Medicine, Sidney Kimmel Comprehensive, Johns Hopkins University, Baltimore, USA

    Robert A. Casero, Jr.

Bibliographic Information

  • Book Title: Polyamines

  • Book Subtitle: Methods and Protocols

  • Editors: Anthony E. Pegg, Robert A. Casero, Jr.

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-034-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-033-1Published: 21 February 2011

  • Softcover ISBN: 978-1-4939-5839-9Published: 23 August 2016

  • eBook ISBN: 978-1-61779-034-8Published: 14 February 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 523

  • Topics: Cell Biology, Laboratory Medicine

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