Methods in Molecular Biology

Signal Transduction Immunohistochemistry

Methods and Protocols

Editors: Kalyuzhny, Alexander E. (Ed.)

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  • Presents detailed, thorough protocols examining this burgeoning field of study
  • Features techniques easily adaptable to numerous research tasks
  • Contains expert notes and vital implementation advice
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  • ISBN 978-1-61779-024-9
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About this book

Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task.  In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC).  Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.

Reviews

From the reviews:

“Each of us daily using immunohistochemical protocols to reveal targets either useful for research or diagnostic aims will surely wonder by which tricky techniques it is possible to overcome the preservation and unmasking of those labile antigens involved in signal transduction. Well, by seventheen chapters grouped in five parts Prof. Alexander E. Kalyuzhny is presenting an invaluable technical and methodological source of hints to satisfy our needs: to overcome troubleshottings if we are already in the field or to orientate those entering the field.” (Carlo Albert Redi, European Journal of Histochemistry, July, 2011)

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies

    Pages 3-43

    Brumbaugh, Kathy (et al.)

  • Selection and Validation of Antibodies for Signal Transduction Immunohistochemistry

    Pages 45-53

    Bodo, Juraj (et al.)

  • An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry

    Pages 55-67

    Kurien, Biji T. (et al.)

  • Optimized Protocol to Make Phospho-Specific Antibodies that Work

    Pages 69-88

    Archuleta, Amy J. (et al.)

  • Methodology and Technology for Stabilization of Specific States of Signal Transduction Proteins

    Pages 91-100

    Borén, Mats

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-61779-024-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
Softcover $179.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Signal Transduction Immunohistochemistry
Book Subtitle
Methods and Protocols
Editors
  • Alexander E. Kalyuzhny
Series Title
Methods in Molecular Biology
Series Volume
717
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-024-9
DOI
10.1007/978-1-61779-024-9
Hardcover ISBN
978-1-61779-023-2
Softcover ISBN
978-1-4939-5804-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 302
Topics