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Methods in Molecular Biology

RNA and DNA Editing

Methods and Protocols

Editors: Aphasizhev, Ruslan (Ed.)

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  • Features cutting-edge methodologies focused on the study of this diverse field of research
  • Includes unique genetic, molecular, biochemical, and computational approaches
  • Contains expert tips and implementation advice
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eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-61779-018-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-61779-017-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-5821-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues.  In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.

Reviews

From the reviews:

“This book on RNA and DNA Editing, Methods and Protocols provides methods to study editing substrates, mechanisms of specificity and functions of RNA and DNA editing enzymes and complexes … . The apparent audience is molecular biologists interested in RNA and DNA editing.” (Omer Iqbal, Doody’s Book Reviews, March, 2012)

“It is quite timely that a Methods book dedicated at experimental approaches to investigate RNA editing mechanisms should find its rightful place in the series. To achieve this aim, the editor … has successfully united many leading experts in this field to write several Chapters dedicated at explaining state-of-the-art techniques … . this is an invaluable store of information that can potentially save weeks or months spent simply in learning what-to-do and what-not-to-do when setting up a particular procedure in their own lab.” (Emanuele Buratti, Molecular Biotechnology, September, 2011)

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Three-Dimensional Reconstruction of Trypanosoma brucei Editosomes Using Single-Particle Electron Microscopy

    Pages 3-22

    Göringer, H. Ulrich (et al.)

  • iCODA: RNAi-Based Inducible Knock-In System in Trypanosoma brucei

    Pages 23-37

    Ringpis, Gene-Errol (et al.)

  • Perturbing A-to-I RNA Editing Using Genetics and Homologous Recombination

    Pages 41-73

    Staber, Cynthia J. (et al.)

  • Laser Microdissection and Pressure Catapulting of Single Human Motor Neurons for RNA Editing Analysis

    Pages 75-87

    Sun, Hui (et al.)

  • Biochemical Identification of A-to-I RNA Editing Sites by the Inosine Chemical Erasing (ICE) Method

    Pages 89-99

    Sakurai, Masayuki (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-61779-018-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-61779-017-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-5821-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
RNA and DNA Editing
Book Subtitle
Methods and Protocols
Editors
  • Ruslan Aphasizhev
Series Title
Methods in Molecular Biology
Series Volume
718
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-018-8
DOI
10.1007/978-1-61779-018-8
Hardcover ISBN
978-1-61779-017-1
Softcover ISBN
978-1-4939-5821-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 275
Topics