Methods in Molecular Biology

Plant Immunity

Methods and Protocols

Editors: McDowell, John M. (Ed.)

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  • Emphasizes emerging technologies in plant immunity
  • Easily applied to a wide variety of plant-microbe interactions
  • Contains expert tips on troubleshooting and key implementation advice
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About this book

A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes.  In  Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens.  These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity.  Written in the highly successful  Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Reviews

From the reviews:

“This volume … describes newly developed or optimised techniques for understanding the immune response of plants and the ways in which pathogens, including the true fungi, oomycetes, nematodes and bacteria, are able to subvert it. … Where appropriate, methods are well illustrated with diagrams, photographs of equipment and expected results. In summary, this is a timely addition to this series and will be of immediate interest and use to all those working in this field.” (Andrew James, Annals of Botany, Vol. 111 (1), January, 2013)


Table of contents (21 chapters)

Table of contents (21 chapters)
  • Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression

    Pages 1-8

    Cournoyer, Patrick (et al.)

  • Fragment Complementation and Co-immunoprecipitation Assays for Understanding R Protein Structure and Function

    Pages 9-20

    Moffett, Peter

  • Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag

    Pages 21-30

    Qi, Yiping (et al.)

  • Biochemical Purification of Native Immune Protein Complexes

    Pages 31-44

    Elmore, James M. (et al.)

  • Chromatin Immunoprecipitation to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity

    Pages 45-58

    Roccaro, Mario (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-61737-998-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-61737-997-0
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-5755-2
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Plant Immunity
Book Subtitle
Methods and Protocols
Editors
  • John M. McDowell
Series Title
Methods in Molecular Biology
Series Volume
712
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
Distribution Rights
Distribution rights for India: CBS Publishers, New Delhi, India
eBook ISBN
978-1-61737-998-7
DOI
10.1007/978-1-61737-998-7
Hardcover ISBN
978-1-61737-997-0
Softcover ISBN
978-1-4939-5755-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 295
Topics