- Emphasizes emerging technologies in plant immunity
- Easily applied to a wide variety of plant-microbe interactions
- Contains expert tips on troubleshooting and key implementation advice
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- About this book
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A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes. In Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens. These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
- Reviews
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From the reviews:
“This volume … describes newly developed or optimised techniques for understanding the immune response of plants and the ways in which pathogens, including the true fungi, oomycetes, nematodes and bacteria, are able to subvert it. … Where appropriate, methods are well illustrated with diagrams, photographs of equipment and expected results. In summary, this is a timely addition to this series and will be of immediate interest and use to all those working in this field.” (Andrew James, Annals of Botany, Vol. 111 (1), January, 2013)
- Table of contents (21 chapters)
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Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression
Pages 1-8
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Fragment Complementation and Co-immunoprecipitation Assays for Understanding R Protein Structure and Function
Pages 9-20
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Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag
Pages 21-30
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Biochemical Purification of Native Immune Protein Complexes
Pages 31-44
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Chromatin Immunoprecipitation to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity
Pages 45-58
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Table of contents (21 chapters)
- Download Preface 1 PDF (1.5 MB)
- Download Sample pages 1 PDF (1.5 MB)
- Download Table of contents PDF (1.4 MB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Plant Immunity
- Book Subtitle
- Methods and Protocols
- Editors
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- John M. McDowell
- Series Title
- Methods in Molecular Biology
- Series Volume
- 712
- Copyright
- 2011
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- Distribution Rights
- Distribution rights for India: CBS Publishers, New Delhi, India
- eBook ISBN
- 978-1-61737-998-7
- DOI
- 10.1007/978-1-61737-998-7
- Hardcover ISBN
- 978-1-61737-997-0
- Softcover ISBN
- 978-1-4939-5755-2
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 295
- Topics
