Methods in Molecular Biology

Regulatory T Cells

Methods and Protocols

Editors: Kassiotis, George, Liston, Adrian (Eds.)

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  • -Provides optimized and standardized protocols to allow reliable and reproducible use
  • -Presents innovations that have lead to insights on the suppressive mechanisms and biology of regulatory T cells
  • -Features tips from the experts to ensure the flawless implementation of the techniques
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eBook $109.00
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  • ISBN 978-1-61737-979-6
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  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
Softcover $149.00
price for USA in USD
About this book

Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses. In Regulatory T Cells: Methods and Protocols, experts in the field to offer a collection of current techniques to advance the study of regulatory T cells, including the use of the IL-2 receptor alpha chain and other markers, as well as the more recently desirable use of the transcription factor FoxP3. Divided into three sections, the book covers, in equal measure, in vitro, in vivo, and human studies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known methodological pitfalls. Authoritative and cutting-edge, Regulatory T Cells: Methods and Protocols distills the most vital current techniques through several years of optimization and standardization in order to allow reliable and reproducible use by both young and experienced cellular and molecular immunologists.

Reviews

From the reviews:

“This book is to provide a detailed and up-to-date collection of methods and protocols utilised for the analysis of regulatory T cells. … this book would be of most use to scientific researchers, in particular PhD students and post-doctoral researchers, working in the field of regulatory T cells within a laboratory setting. For individuals using in vitro or in vivo mouse models for regulatory T cell analysis, this book would be a perfect asset.” (Karen Smith, Immunology News, February, 2012)

“This volume in the Methods in Molecular Biology series is a collection of current techniques to study regulatory T cells. … Researchers who need to discover techniques to isolate or use regulatory T cells are the primary audience. The authors of the individual chapters are knowledgeable about the techniques they describe. … There are other books that discuss regulatory T cells, but not with the level of procedural detail that this one has. It would be a useful reference for a library … .” (Marion C. Cohen, Doody's Book Reviews, August, 2011)

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Regulatory T Cells: History and Perspective

    Pages 3-17

    Sakaguchi, Shimon

  • In Vitro Treg Suppression Assays

    Pages 21-37

    Collison, Lauren W. (et al.)

  • Generation of T Cell Hybridomas from Naturally Occurring FoxP3+ Regulatory T Cells

    Pages 39-44

    Singh, Nagendra (et al.)

  • In Vitro and In Vivo Analyses of Regulatory T Cell Suppression of CD8+ T Cells

    Pages 45-54

    Hasenkrug, Kim J. (et al.)

  • Flow Cytometric Profiling of Mature and Developing Regulatory T Cells in the Thymus

    Pages 55-69

    Simons, Donald M. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-61737-979-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
Softcover $149.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Regulatory T Cells
Book Subtitle
Methods and Protocols
Editors
  • George Kassiotis
  • Adrian Liston
Series Title
Methods in Molecular Biology
Series Volume
707
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61737-979-6
DOI
10.1007/978-1-61737-979-6
Hardcover ISBN
978-1-61737-978-9
Softcover ISBN
978-1-4939-6158-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 282
Topics