Methods in Molecular Biology

Cell-Based Microarrays

Methods and Protocols

Editors: Palmer, Ella (Ed.)

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  • Provides an up-to-date variety of protocols covering all aspects in thorough, clear detail
  • Presents readers with clear step-by-step protocols to allow them to set up difficult techniques and expand upon them
  • Includes tips from the experts highlighting potential methodological pitfalls in order to ensure successful implementation
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  • ISBN 978-1-61737-970-3
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Hardcover $149.99
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Softcover $149.00
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About this book

As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Cell-Based Microarrays: Overview

    Pages 1-12

    Palmer, Ella

  • Cell-Based Co-transfection Microarrays for Use with HEK293T Cells on a Poly d-Lysine-Coated Polystyrene Microplate

    Pages 13-25

    Soni, Meenal (et al.)

  • Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays

    Pages 27-40

    Palmer, Ella (et al.)

  • A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays

    Pages 41-52

    Redmond, Tanya M. (et al.)

  • High-Throughput Subcellular Protein Localization Using Transfected-Cell Arrays

    Pages 53-72

    Hu, Yuhui (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-61737-970-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-61737-969-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-5784-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cell-Based Microarrays
Book Subtitle
Methods and Protocols
Editors
  • Ella Palmer
Series Title
Methods in Molecular Biology
Series Volume
706
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61737-970-3
DOI
10.1007/978-1-61737-970-3
Hardcover ISBN
978-1-61737-969-7
Softcover ISBN
978-1-4939-5784-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 209
Topics